College of Pharmacy, Dali University, Dali, 671000, China.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.
Phytochemistry. 2023 Dec;216:113882. doi: 10.1016/j.phytochem.2023.113882. Epub 2023 Oct 4.
Schisandra chinensis is an important traditional Chinese medicine and its main bioactive components are lignans and schinortriterpenoids (SNTs). The aim of this study was to explore the biologically rich SNTs from the stem and leaves of S. chinensis (SCSL). Here, seven previously undescribed 7/8/5 and 7/8/3 carbon skeleton SNTs (1-7) were reported. Their structures were determined by NMR, UV, MS, ECD, and X-ray diffraction analyses, and the neuroprotective activities of these compounds on corticosterone-induced PC12 cell injury were evaluated. The results showed that 1, 5, and 7 (25 μM) had neuroprotective effects, and the cell viability was increased by 20.07%, 14.24%, and 15.14% (positive control: 30.64%), respectively. These findings increased the number of described SNTs in SCSL, and the neuroprotective activities of all compounds indicated their potential application in neurodegenerative diseases.
北五味子是一种重要的中药，其主要生物活性成分为木脂素和五味子三萜（SNTs）。本研究旨在从北五味子的茎和叶中探索具有丰富生物活性的 SNTs。在此，报道了 7 个以前未描述的具有 7/8/5 和 7/8/3 个碳骨架的 SNTs（1-7）。通过 NMR、UV、MS、ECD 和 X 射线衍射分析确定了它们的结构，并评估了这些化合物对皮质酮诱导的 PC12 细胞损伤的神经保护活性。结果表明，1、5 和 7（25μM）具有神经保护作用，细胞活力分别增加了 20.07%、14.24%和 15.14%（阳性对照：30.64%）。这些发现增加了 SCSL 中描述的 SNTs 的数量，所有化合物的神经保护活性表明它们在神经退行性疾病中的潜在应用。
