Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China.
Henan Academy of Fishery Sciences, Zhengzhou, 450044, People's Republic of China.
Fish Shellfish Immunol. 2023 Nov;142:109135. doi: 10.1016/j.fsi.2023.109135. Epub 2023 Oct 4.
The purpose of this experiment was to study the mitigation effect of sulforaphane (SFN) on fish toxicological damage caused by triphenyltin (TPT) pollution. A total of 320 healthy fish (56.9 ± 0.4g) were randomly placed into four groups, each with four duplicates. The control group was fed the basal diet, the TPT group was exposed to 10 ng/L TPT on the basis of the control group, the SFN group was fed a diet supplemented with 10 mg/kg SFN, the SFN + TPT group was exposed to 10 ng/L TPT on the basis of the SFN group. Each tank had 20 fish and the breeding lasted for 8 weeks. The present study found that the antioxidant enzyme activity in the TPT group was significantly lower than that of the control group (P < 0.05). In addition, compared with the control group, the mRNA expression of pro-inflammatory factors (IL-6, TNF-α) were significantly induced, and the anti-inflammatory factor genes (IL-10, TGF) were significantly inhibited (P < 0.05) in TPT group. SFN relieved the changes of inflammatory factors caused by TPT, ameliorated oxidative stress, improved antioxidant enzyme (include SOD, CAT, GSH, GPx) activities (P < 0.05). 16s RNA analysis indicated that exposure to TPT caused changes in intestinal microflora. The results of the study showed that after exposure to TPT, some beneficial genera of bacteria in the gut of Rhizobiaceae, Bdellovibrio and Candidatus Alysiosphaera were decreased. The bacteria associated with intestinal inflammation including Propionibacterium, Rubrobacter, Anaerorhabdus_furcosa_group, Rikenellaceae and Eubacterium_brachy were upregulated. However, the SFN treatment group significantly down-regulated the above five inflammation-related bacteria. The above results indicated that TPT caused oxidative stress and inflammation in fish intestines, changed the intestinal microflora, and dietary SFN could improve antioxidant status, regulate inflammation and intestinal health. Therefore, SFN is a promising diet additive for improving fish damage caused by TPT contamination.
本实验旨在研究萝卜硫素（SFN）对三苯基锡（TPT）污染对鱼类毒理学损伤的缓解作用。将 320 条健康的鱼（56.9±0.4g）随机分为四组，每组四个重复。对照组投喂基础日粮，TPT 组在对照组基础上暴露于 10ng/L TPT，SFN 组投喂添加 10mg/kg SFN 的日粮，SFN+TPT 组在 SFN 组基础上暴露于 10ng/L TPT。每个鱼缸有 20 条鱼，养殖持续 8 周。本研究发现，TPT 组抗氧化酶活性明显低于对照组（P<0.05）。此外，与对照组相比，TPT 组促炎因子（IL-6、TNF-α）的 mRNA 表达明显上调，抗炎因子基因（IL-10、TGF）明显受抑制（P<0.05）。SFN 缓解了 TPT 引起的炎症因子变化，改善了氧化应激，提高了抗氧化酶（包括 SOD、CAT、GSH、GPx）活性（P<0.05）。16s RNA 分析表明，暴露于 TPT 导致肠道微生物群发生变化。研究结果表明，暴露于 TPT 后，肠道中 Rhizobiaceae、Bdellovibrio 和 Candidatus Alysiosphaera 等有益细菌的一些有益属减少。与肠道炎症相关的细菌，包括 Propionibacterium、Rubrobacter、Anaerorhabdus_furcosa_group、Rikenellaceae 和 Eubacterium_brachy 等，上调。然而，SFN 处理组显著下调了上述五种与炎症相关的细菌。上述结果表明，TPT 导致鱼类肠道氧化应激和炎症，改变了肠道微生物群，膳食 SFN 可以改善抗氧化状态，调节炎症和肠道健康。因此，SFN 是一种有前途的饮食添加剂，可改善 TPT 污染对鱼类造成的损伤。
