Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China.
Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China.
Ecotoxicol Environ Saf. 2024 Oct 1;284:116882. doi: 10.1016/j.ecoenv.2024.116882. Epub 2024 Aug 21.
This study aimed to investigate the protective effect of sulforaphane (SFN) on liver injury induced by triphenyltin (TPT) in Cyprinus carpio (C. carpio). The fish (average weight of 56.9±0.4 g) were divided into 4 groups with four replicates: the control, TPT, SFN+TPT and SFN groups. Twenty fish were selected from each tank and cultured for 8 weeks. Then, serum and liver samples were collected for physiological, biochemical and metabolomic analyses. In the present study, TPT downregulated the expression of the lysozyme gene, upregulated HSP70 and Hsp90 gene expression, and decreased the activities of serum antioxidant enzymes (SOD, CAT, and GPX). However, dietary SFN alleviated oxidative stress, and prevented changes in immune genes. Metabolomic analysis revealed that TPT exposure changed key metabolites in the main phenylalanine, fatty acid and glycerophosphatide metabolic pathways, which are related to inflammation, oxidative stress and immunity and might also lead to an imbalance of liver energy and lipid metabolism. Dietary SFN promoted amino acid metabolism and increased metabolites related to immunity, anti-inflammation, antioxidation, and protein synthesis in liver of C. carpio. In summary, dietary SFN supplementation reversed TPT-induced decreases in immunity and oxidative stress and regulated amino acid metabolism, lipid metabolism, inflammation and immunity-related metabolic pathways.
本研究旨在探究萝卜硫素（SFN）对三苯基锡（TPT）诱导的鲤鱼（Cyprinus carpio）肝损伤的保护作用。将鱼（平均体重 56.9±0.4g）分为 4 组，每组 4 个重复：对照组、TPT 组、SFN+TPT 组和 SFN 组。每组从每个水槽中选择 20 条鱼进行 8 周的养殖。然后，采集血清和肝脏样本进行生理、生化和代谢组学分析。本研究中，TPT 下调溶菌酶基因的表达，上调 HSP70 和 Hsp90 基因的表达，并降低血清抗氧化酶（SOD、CAT 和 GPX）的活性。然而，膳食 SFN 缓解了氧化应激，并防止了免疫基因的变化。代谢组学分析表明，TPT 暴露改变了主要苯丙氨酸、脂肪酸和甘油磷脂代谢途径中的关键代谢物，这些代谢物与炎症、氧化应激和免疫有关，也可能导致肝脏能量和脂质代谢失衡。膳食 SFN 促进了氨基酸代谢，并增加了肝脏中与免疫、抗炎、抗氧化和蛋白质合成相关的代谢物。总之，膳食 SFN 补充剂逆转了 TPT 诱导的免疫和氧化应激降低，并调节了氨基酸代谢、脂质代谢、炎症和免疫相关代谢途径。
