Faculty of Pharmacy, Aqaba University of Technology, Aqaba, 11191, Jordan.
Department of Chemical Sciences, Faculty of Applied Sciences, South Eastern University, Oluvil, Sri Lanka.
BMC Res Notes. 2023 Oct 6;16(1):260. doi: 10.1186/s13104-023-06523-8.
Natural products such as terpenoidal compounds have been extremely tested against pathogenic bacteria. Researches are frequently carried out to find out new natural, semisynthetic and synthetic antibacterial agents due to problems of resistance. Thymoquinone derivative was obtained in our previous study and the current research is a continuation. The antibacterial activity of a monoterpenoid; thymoquinone derivative, 5-isopropyl-2-methyloxepine-1-one (1) has been evaluated for the first time by following the Agar cup bioassay method employed. The bacterial strains used in this study were Escherichia coli and Bacillus subtilis. Compound 1 showed moderate activity against Gram-positive organism; B. subtilis and good activity against Gram-negative species; E. coli with zones of inhibition (ZOI) 10.0 ± 0.2 mm and 11.0 ± 0.2 mm against E. coli and B. subtilis, respectively, and in comparison with antibiotic, imipenem. The zones of inhibition were calculated as the mean of the triplicate. The antibacterial activity of thymoquinone derivative 1 could be explained by the presence of unsaturated lactone.
天然产物，如萜类化合物，已经被广泛测试用于对抗致病菌。由于耐药性问题，人们经常进行研究以寻找新的天然、半合成和合成抗菌剂。我们之前的研究中获得了百里醌衍生物，目前的研究是在此基础上的延续。通过琼脂杯生物测定法，首次评估了单萜类化合物——5-异丙基-2-甲基恶唑啉-1-酮（1）的抗菌活性。本研究中使用的细菌菌株为大肠杆菌和枯草芽孢杆菌。化合物 1 对革兰氏阳性菌枯草芽孢杆菌表现出中等活性，对革兰氏阴性菌大肠杆菌表现出良好的活性，对大肠杆菌和枯草芽孢杆菌的抑菌圈直径（ZOI）分别为 10.0±0.2 毫米和 11.0±0.2 毫米，与抗生素亚胺培南相比。抑菌圈直径是三个重复实验的平均值。1 号百里醌衍生物的抗菌活性可以用不饱和内酯的存在来解释。
