Shakir Sara, Mubin Muhammad, Nahid Nazia, Serfraz Saad, Qureshi Muhammad Amir, Lee Taek-Kyun, Liaqat Iram, Lee Sukchan, Nawaz-Ul-Rehman Muhammad Shah
Plant Genetics Lab, Gembloux Agro-Bio Tech, University of Liѐge, Gembloux, Belgium.
Virology Lab, Center for Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Faisalabad, Pakistan.
Front Microbiol. 2023 Sep 20;14:1224221. doi: 10.3389/fmicb.2023.1224221. eCollection 2023.
Circular single-stranded DNA viruses of the family encode replication-associated protein (Rep), which is a multifunctional protein involved in virus DNA replication, transcription of virus genes, and suppression of host defense responses. Geminivirus genomes are replicated through the interaction between virus Rep and several host proteins. The Rep also interacts with itself and the virus replication enhancer protein (REn), which is another essential component of the geminivirus replicase complex that interacts with host DNA polymerases α and δ. Recent studies revealed the structural and functional complexities of geminivirus Rep, which is believed to have evolved from plasmids containing a signature domain (HUH) for single-stranded DNA binding with nuclease activity. The Rep coding sequence encompasses the entire coding sequence for AC4, which is intricately embedded within it, and performs several overlapping functions like Rep, supporting virus infection. This review investigated the structural and functional diversity of the geminivirus Rep.
该科的环状单链DNA病毒编码复制相关蛋白（Rep），它是一种多功能蛋白，参与病毒DNA复制、病毒基因转录以及宿主防御反应的抑制。双生病毒基因组通过病毒Rep与几种宿主蛋白之间的相互作用进行复制。Rep还与自身以及病毒复制增强蛋白（REn）相互作用，REn是双生病毒复制酶复合体的另一个重要组成部分，与宿主DNA聚合酶α和δ相互作用。最近的研究揭示了双生病毒Rep的结构和功能复杂性，据信它是从含有用于单链DNA结合且具有核酸酶活性的特征结构域（HUH）的质粒进化而来的。Rep编码序列包含AC4的整个编码序列，AC4复杂地嵌入其中，并执行与Rep类似的几种重叠功能，支持病毒感染。本综述研究了双生病毒Rep的结构和功能多样性。
