Hillyer G V, Gómez de Rios I
Am J Trop Med Hyg. 1979 Mar;28(2):237-41. doi: 10.4269/ajtmh.1979.28.237.
The enzyme-linked immunosorbent assay (ELISA) done with soluble egg antigens (SEA) of Schistosoma mansoni was utilized for the detection of infections with schistosomes. The method readily detected experimental infections in NIH outbred, New Zealand black, and New Zealand white mice by 6-10 weeks post-exposure to S. mansoni cercariae. In addition, the test was negative when the sera from 11 Puerto Rican normal controls were examined and was positive in 8 of 10 serum samples from humans with schistosomiasis mansoni. However, extensive cross-reactivity was seen when using serum from humans with fascioliasis, trichinosis, cysticercosis, and echinococcosis. Thus the ELISA test done with SEA as antigen lacks immunologic specificity. For the method to be an effective seroepidemiological tool in areas where these parasites are endemic further purification of the antigen and more extensive understanding of its components are needed.
利用曼氏血吸虫可溶性虫卵抗原（SEA）进行的酶联免疫吸附测定（ELISA）用于检测血吸虫感染。该方法在暴露于曼氏血吸虫尾蚴后6 - 10周，能轻易检测出美国国立卫生研究院远交群小鼠、新西兰黑鼠和新西兰白鼠的实验性感染。此外，检测11名波多黎各正常对照者的血清时，该试验呈阴性，而在10名曼氏血吸虫病患者的血清样本中有8份呈阳性。然而，当使用来自患有肝片吸虫病、旋毛虫病、囊尾蚴病和棘球蚴病患者的血清时，出现了广泛的交叉反应。因此，以SEA作为抗原的ELISA试验缺乏免疫特异性。要使该方法成为这些寄生虫流行地区有效的血清流行病学工具，需要进一步纯化抗原并更深入了解其成分。
