Derouin F, Heyer F, Lariviere M, Petithory J
Pathol Biol (Paris). 1980 Sep;28(7):465-8.
Sera from patients with schistosomiasis and various infections were examined by an enzyme-linked immunosorbant assay technique (ELISA) using soluble antigens prepared from adult worms and eggs of Schistosoma mansoni. Marked false positive reactions were observed in cases of certain parasitic (hydatidosis) and non parasitic diseases (liver cirrhosis). Equivalent results with the techniques of immunofluorescence and immunoenzymology (done on adult sections) were obtained with adult worm antigen read at a higher optic-density limit. At present, this technique might be useful in seroepidemiological surveys; however, further purification of the antigen will increase its sensibility and specificity.
采用从曼氏血吸虫成虫和虫卵制备的可溶性抗原，通过酶联免疫吸附测定技术（ELISA）对血吸虫病患者及各种感染者的血清进行检测。在某些寄生虫病（包虫病）和非寄生虫病（肝硬化）病例中观察到明显的假阳性反应。用免疫荧光技术和免疫酶技术（对成虫切片进行检测），以较高的光密度极限读取成虫抗原，得到了相同的结果。目前，这项技术可能在血清流行病学调查中有用；然而，进一步纯化抗原将提高其敏感性和特异性。
