Jilin Agricultural University, College of Resources and Environmental Science/College of Plant Protection/Key Laboratory of Sustainable Utilization of Soil Resources in Commodity Grain Base of Jilin Province/Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, Ministry of Education, Changchun 130118, China.
Jilin Agricultural University, College of Resources and Environmental Science/College of Plant Protection/Key Laboratory of Sustainable Utilization of Soil Resources in Commodity Grain Base of Jilin Province/Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, Ministry of Education, Changchun 130118, China.
Ecotoxicol Environ Saf. 2023 Oct 15;265:115539. doi: 10.1016/j.ecoenv.2023.115539. Epub 2023 Oct 4.
Nitrification inhibitors (NIs) have been widely applied to inhibit nitrification and reduce NO emissions in agriculture. However, there are still some shortcomings, e.g. short effective periods, large applying amounts, low effectiveness, easy deactivation and different effect. Thus, a nitrapyrin microcapsule suspension (CPCS) was used as a new experimental material to elaborate its effects on nitrogen transformation and microbial response mechanisms in black soil by cultivation experiments with six treatments of no fertilization (CK), urea, urea+ 0.2 % CPES, urea+ 0.1 % CPCS, urea+ 0.2 % CPCS, and urea+ 0.3 % CPCS. The content of ammonium, nitrate nitrogen, functional microbial activity, degradation rate and adsorption characteristics of CPCS in the soil at different incubating times were determine. Compared with the nitrapyrin emulsifiable concentrate (CPEC) treatment, the degradation rate of CPCS decreased by 21.54 %, the half-life increased by 10.2 days, and the adsorption rate of nitrapyrin on black soil decreased more than 6-fold. CPCS effectively inhibited the transformation of ammonium nitrogen to nitrate nitrogen within more than 42 days. CPCS had a negative effect on amoA gene abundance and a positive effect on nrfA gene abundance. The research results provide a basic theoretical support for the application of CPCS on black soil.
硝化抑制剂（NIs）已广泛应用于农业领域，以抑制硝化作用并减少 NO 排放。然而，它们仍存在一些缺点，如有效期短、施用量大、效果低、易失活以及作用效果不同等。因此，本文采用硝化抑制微胶囊悬浮剂（CPCS）作为一种新型试验材料，通过 6 种处理（不施肥对照 CK、尿素、尿素+0.2% CPES、尿素+0.1% CPCS、尿素+0.2% CPCS 和尿素+0.3% CPCS）的培养试验，研究其在黑土中对氮素转化及微生物响应机制的影响。测定了不同培养时间下土壤中铵态氮、硝态氮含量、功能微生物活性、CPCS 降解率及吸附特征。与硝呋草腙乳油（CPEC）处理相比，CPCS 的降解率降低了 21.54%，半衰期延长了 10.2 天，黑土对硝呋草腙的吸附率降低了 6 倍以上。CPCS 能有效抑制铵态氮向硝态氮的转化，持续 42 天以上。CPCS 对 amoA 基因丰度有负向影响，对 nrfA 基因丰度有正向影响。本研究结果为 CPCS 在黑土中的应用提供了基本的理论支持。
