Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
Ningxia Innovation Center of Goji R & D, Yinchuan 750002, PR China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 5):127256. doi: 10.1016/j.ijbiomac.2023.127256. Epub 2023 Oct 5.
At present, the structure-activity relationship of polysaccharides is a common and important focus in the fields of glycobiology and carbohydrate chemistry. To better understand the effect of specific polysaccharide structures on bioactive orientation, four homogeneous polysaccharides from Lycii fructus, one neutral along with three acidic polysaccharides, were purified, structurally characterized and comparatively evaluated on the antioxidative and anti-aging activities. The GC-MS-based monosaccharide composition analysis and methylation results showed that the LFPs had similar glycosyl types but varied proportions. Nuclear magnetic resonance (NMR) spectroscopy showed that LFPs consisted of arabinogalactan, rhamnogalacturonan and homogalacturonan structural domains. The results of the structure-activity relationship indicated that the antioxidative activity was positively correlated with the galacturonic acid (GalA) content, while the neutral multi-branched chains might be responsible for the anti-aging activity. This study is the first time to compare the principal structures and multiple biological activities of LFPs, which provided a reference for the industrial development and deep excavation of the health value of LFPs.
目前，多糖的结构-活性关系是糖生物学和碳水化合物化学领域的一个共同且重要的焦点。为了更好地了解特定多糖结构对生物活性定向的影响，从枸杞中纯化了四种均一多糖，一种中性和三种酸性多糖，并对其抗氧化和抗衰老活性进行了结构表征和比较评价。基于 GC-MS 的单糖组成分析和甲基化结果表明，LFPs 具有相似的糖苷类型，但比例不同。核磁共振（NMR）光谱表明，LFPs 由阿拉伯半乳聚糖、鼠李半乳糖醛酸聚糖和同质半乳糖醛酸聚糖结构域组成。结构-活性关系的结果表明，抗氧化活性与半乳糖醛酸（GalA）含量呈正相关，而中性多支链可能负责抗衰老活性。本研究首次比较了 LFPs 的主要结构和多种生物学活性，为 LFPs 的工业发展和健康价值的深入挖掘提供了参考。
