Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, PR China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, PR China; School of Pharmacy, Ningxia Medical University, Yinchuan, 750004, PR China; Key Laboratory of Hui Ethnic Medicine Modernization of Ministry of Education, Yinchuan, 750004, PR China.
Carbohydr Polym. 2020 Dec 1;249:116894. doi: 10.1016/j.carbpol.2020.116894. Epub 2020 Aug 12.
Regeneration of neurites network constitutes a neurotrophic and therapeutic strategy for Parkinson's disease (PD). Increasing evidence is supporting the potential application of natural polysaccharides in prevention or treatment of PD. In this study, an acidic heteropolysaccharide LFP-1 was isolated from Lycii fructus, and purified by ion-exchange and gel filtration chromatography. Structural features of LFP-1 were analyzed with molecular weight (MW) distribution, monosaccharide composition, methylation and nuclear magnetic resonance (NMR) spectra. LFP-1 was a complicated structured polysaccharide with an average MW of 1.78 × 10 Da and composed of highly branched arabinogalactans, homogalacturonan and rhamnogalacturonan moieties. LFP-1 promoted neuronal differentiation and neurite outgrowth in vitro in PC12 cell models. Furthermore, LFP-1 had a significantly protective effect against 1-methyl-4-phenylpyridiniumion (MPP+)-induced neurotoxicity in PD model PC12 cells. These observations unambiguously indicated the neurotrophic and neuroprotective activities of LFP-1, which may be developed for prevention or treatment of neurodegeneration in PD.
神经突网络的再生是治疗帕金森病（PD）的神经营养和治疗策略。越来越多的证据支持天然多糖在预防或治疗 PD 中的潜在应用。本研究从枸杞中分离出一种酸性杂多糖 LFP-1，并通过离子交换和凝胶过滤色谱进行纯化。用分子量（MW）分布、单糖组成、甲基化和核磁共振（NMR）谱分析 LFP-1 的结构特征。LFP-1 是一种结构复杂的多糖，平均 MW 为 1.78×10 Da，由高度分支的阿拉伯半乳聚糖、同质半乳糖醛酸和鼠李半乳糖醛酸组成。LFP-1 在体外 PC12 细胞模型中促进神经元分化和神经突生长。此外，LFP-1 对 1-甲基-4-苯基吡啶鎓（MPP+）诱导的 PD 模型 PC12 细胞神经毒性具有显著的保护作用。这些观察结果明确表明了 LFP-1 的神经营养和神经保护活性，这可能为预防或治疗 PD 中的神经退行性变提供了一种可能。
