Callen D F, Hyland V J, Baker E G, Fratini A, Simmers R N, Mulley J C, Sutherland G R
Department of Histopathology, Adelaide Children's Hospital.
Genomics. 1988 Feb;2(2):144-53. doi: 10.1016/0888-7543(88)90096-1.
The fragile site, FRA16B, at 16q22.100 and four different translocations with breakpoints at 16q22.102, 16q22.105, 16q22.108, and 16q22.3 were used to locate and order DNA probes. This was achieved by Southern analysis of a somatic cell hybrid panel containing portions of chromosome 16 and by in situ hybridization. The anonymous DNA fragments D16S6, D16S10, and D16S11 were proximal to FRA16B and located at 16q13----q22.100. D16S4 and LCAT were located at 16q22.100----q22.102. TAT and HP were located at 16q22.105----q22.108. CTRB was located distal to 16q22.105 and therefore is in the distal half of 16q22. The order of markers in this region was determined as centromere-D16S6, D16S11, D16S10, MT-FRA16B-D16S4, LCAT-HP,TAT,CTRB-APRT- telomere. Linkage studies to determine map distances between the closest markers flanking the fragile site are now in progress.
位于16q22.100的脆性位点FRA16B以及断点位于16q22.102、16q22.105、16q22.108和16q22.3的四种不同易位用于定位和排列DNA探针。这是通过对包含16号染色体部分片段的体细胞杂交板进行Southern分析以及原位杂交来实现的。匿名DNA片段D16S6、D16S10和D16S11位于FRA16B近端，定位于16q13----q22.100。D16S4和LCAT位于16q22.100----q22.102。TAT和HP位于16q22.105----q22.108。CTRB位于16q22.105远端，因此位于16q22的后半部分。该区域标记的顺序确定为着丝粒-D16S6、D16S11、D16S10、MT-FRA16B-D16S4、LCAT-HP、TAT、CTRB-APRT-端粒。目前正在进行连锁研究以确定脆性位点两侧最接近标记之间的图谱距离。
