Zhang Yongjin, Wang Ju, Ge Wupeng, Song Yuxuan, He Rui, Wang Zhi, Zhao Lili
College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
Shaanxi Engineering Research Centre of Dairy Products Quality, Safety and Health, Northwest A&F University, Yangling 712100, China.
Food Res Int. 2023 Nov;173(Pt 1):113278. doi: 10.1016/j.foodres.2023.113278. Epub 2023 Jul 13.
This study aimed to investigate the hypoglycemic effect of Camel milk peptides (CMPs) on Type 2 diabetes mellitus (T2DM) mice and reveal its related mechanism from the aspect of gut microbiota and metabolites. The administering CMPs significantly alleviated the weight loss, polydipsia and polyphagia, reduced fasting blood glucose (FBG), improved insulin resistance and sensitivity, and restored the level of serum hormones, lipopolysaccharide (LPS), lipid metabolic and tissue damage. Furthermore, CMPs intervention remarkably reversed gut microbiota dysbiosis in T2DM mice by reducing the relative abundance of Proteobacteria, Allobaculum, Clostridium, Shigella and the Firmicutes/Bacteroidetes ratio, while increasing the relative abundance of Bacteroidetes and Blautia. Metabolomic analysis identified 84 different metabolites between T2DM and CMPs-treated groups, participating in three pathways of Pantothenate and CoA biosynthesis, Phenylalanine metabolism and Linoleic acid metabolism. Ureidopropionic acid, pantothenic acid, hippuric acid, hydrocinnamic acid and linoleic acid were identified as key acidic metabolites closely related to hypoglycemic effect. Correlation analysis indicated that CMPs might have a hypoglycemic effect through their impact on gut microbiota, leading to variations in short-chain fatty acids (SCFAs), acidic metabolites and metabolic pathways. These findings suggest that CMPs could be a beneficial nutritional supplement for intervention T2DM.
本研究旨在探讨骆驼奶肽（CMPs）对2型糖尿病（T2DM）小鼠的降血糖作用，并从肠道微生物群和代谢产物方面揭示其相关机制。给予CMPs可显著减轻体重减轻、多饮和多食症状，降低空腹血糖（FBG），改善胰岛素抵抗和敏感性，并恢复血清激素、脂多糖（LPS）、脂质代谢和组织损伤水平。此外，CMPs干预通过降低变形菌门、嗜胆菌属、梭菌属、志贺氏菌属的相对丰度以及厚壁菌门/拟杆菌门的比例，同时增加拟杆菌门和布劳特氏菌属的相对丰度，显著逆转了T2DM小鼠肠道微生物群的失调。代谢组学分析确定了T2DM组和CMPs治疗组之间84种不同的代谢产物，它们参与泛酸和辅酶A生物合成、苯丙氨酸代谢和亚油酸代谢三条途径。脲基丙酸、泛酸、马尿酸、氢化肉桂酸和亚油酸被确定为与降血糖作用密切相关的关键酸性代谢产物。相关性分析表明，CMPs可能通过影响肠道微生物群而产生降血糖作用，导致短链脂肪酸（SCFAs）、酸性代谢产物和代谢途径的变化。这些发现表明，CMPs可能是干预T2DM的有益营养补充剂。
