Department of Ophthalmology, The First Affiliated Hospital of Xinjiang Medical University, Urumchi, China.
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumchi, China.
Curr Eye Res. 2024 Jan;49(1):80-87. doi: 10.1080/02713683.2023.2268306. Epub 2024 Jan 3.
To study the systemic inflammatory mediator levels in non-proliferative diabetic retinopathy (NPDR) patients with diabetic macular edema (DME) and explore the correlation between systemic inflammatory mediators and DME.
In this prospective study, we included 25 patients without diabetes (control group) and 75 patients with type 2 diabetes mellitus (diabetic group). According to fundus examination, the diabetic group patients were divided into: diabetic patients without diabetic retinopathy (DR) (Non-DR group), NPDR patients without DME (Non-DME group), and NPDR patients with DME (DME group). Serum levels of a broad panel of inflammatory mediators were analysed by multiplex protein quantitative detection technology based on a flow cytometry detection system.
The interferon-γ (IFN-γ) levels were significantly higher in DME group and Non-DME group as compared to control group ( = 0.023 and = 0.033) and Non-DR group ( = 0.009 and = 0.015). Significantly higher values were obtained in DME group and Non-DME group as compared to control group for the interleukin-8 (IL-8) ( = 0.003 and = 0.003). The IL-23 levels were significantly elevated in DME group and Non-DR group than in Non-DME group ( = 0.013 and = 0.004). The diabetic group had significantly higher serum levels of IL-8 and IL-33 ( = 0.001 and = 0.011), and lower serum levels of tumor necrosis factor-α (TNF-α) ( = 0.027) in comparison with control group.
The changed levels of serum inflammatory mediators suggest that the systemic inflammatory mediators are involved in the pathogenesis of NPDR patients with DME. Such effects can guide clinical monitoring, diagnostic and therapeutic approaches for DME patients at an early stage.
研究非增生性糖尿病性视网膜病变（NPDR）合并糖尿病性黄斑水肿（DME）患者的全身炎症介质水平，并探讨全身炎症介质与 DME 的相关性。
本前瞻性研究纳入 25 例无糖尿病患者（对照组）和 75 例 2 型糖尿病患者（糖尿病组）。根据眼底检查，糖尿病组患者分为：无糖尿病视网膜病变（DR）的糖尿病患者（非 DR 组）、无 DME 的 NPDR 患者（非 DME 组）和 DME 的 NPDR 患者（DME 组）。采用基于流式细胞检测系统的多重蛋白定量检测技术分析血清中广泛炎症介质的水平。
与对照组相比，DME 组和非 DME 组的干扰素-γ（IFN-γ）水平明显升高（=0.023 和=0.033），而非 DR 组也明显升高（=0.009 和=0.015）。与对照组相比，DME 组和非 DME 组的白细胞介素-8（IL-8）水平明显升高（=0.003 和=0.003）。DME 组和非 DR 组的白细胞介素-23（IL-23）水平明显高于非 DME 组（=0.013 和=0.004）。与对照组相比，糖尿病组血清白细胞介素-8 和白细胞介素-33 水平明显升高（=0.001 和=0.011），肿瘤坏死因子-α（TNF-α）水平明显降低（=0.027）。
血清炎症介质水平的变化提示全身炎症介质参与 NPDR 合并 DME 患者的发病机制。这些作用可以指导 DME 患者早期的临床监测、诊断和治疗方法。
