Department of Ophthalmology, GMC, Amritsar, Punjab, India.
Department of Biochemistry, GMC, Amritsar, Punjab, India.
Indian J Ophthalmol. 2023 Jun;71(6):2521-2525. doi: 10.4103/IJO.IJO_345_23.
To determine the correlation between serum inflammatory and metabolic biomarkers of patients with diabetic retinopathy (DR) and diabetic macular edema (DME).
Serum samples were obtained from 100 diabetic patients. Patients were divided into three groups: group 1 (patients with no DR, n = 27), group 2 (DR with DME, n = 34), and group 3 (DR without DME, n = 39). Serum concentrations of C-reactive protein (CRP) and interleukin-6 (IL-6) were measured by quantitative turbidimetric immunoassay and sandwich chemiluminescence immunoassay, respectively. Metabolic parameters such as glycated hemoglobin (HbA1c), total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), serum creatinine, and blood urea were determined by automated analyzer om-360 after standardization.
The levels of IL-6 and CRP differed significantly in patients with DR and without DR (P < 0.001 and P = 0.045, respectively). We also found a positive correlation between IL-6 and CRP with the severity of DR. When DR patients with DME were compared to patients without DME, only IL-6 was observed to be significantly elevated (P < 0.001). None of the metabolic markers correlated significantly with DR and DME.
Significantly raised levels of serum inflammatory biomarkers can be used to elucidate the significant role of inflammation in the pathogenesis of DR. Therefore, circulating biomarkers can serve as diagnostic and therapeutic predictors for monitoring the onset and progression of DR and DME.
确定患有糖尿病视网膜病变（DR）和糖尿病黄斑水肿（DME）的患者血清炎症和代谢生物标志物之间的相关性。
从 100 名糖尿病患者中获得血清样本。患者分为三组：第 1 组（无 DR，n = 27），第 2 组（DR 伴 DME，n = 34）和第 3 组（DR 无 DME，n = 39）。通过定量比浊免疫测定法和夹心化学发光免疫测定法分别测量血清 C 反应蛋白（CRP）和白细胞介素 6（IL-6）的浓度。通过标准化后的 om-360 自动分析仪测定糖化血红蛋白（HbA1c）、总胆固醇、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、甘油三酯（TG）、血清肌酐和血尿素等代谢参数。
DR 患者和无 DR 患者的 IL-6 和 CRP 水平差异有统计学意义（P < 0.001 和 P = 0.045）。我们还发现 IL-6 和 CRP 与 DR 的严重程度呈正相关。与无 DME 的 DR 患者相比，仅观察到 IL-6 显著升高（P < 0.001）。代谢标志物与 DR 和 DME 均无显著相关性。
血清炎症生物标志物的显著升高可用于阐明炎症在 DR 发病机制中的重要作用。因此，循环生物标志物可作为诊断和治疗预测因子，用于监测 DR 和 DME 的发生和进展。
