Ariute Juan Carlos, Coelho-Rocha Nina Dias, Dantas Carlos Willian Dias, de Vasconcelos Larissa Amorim Tourinho, Profeta Rodrigo, de Jesus Sousa Thiago, de Souza Novaes Ane, Galotti Bruno, Gomes Lucas Gabriel, Gimenez Enrico Giovanelli Toccani, Diniz Carlos, Dias Mariana Vieira, de Jesus Luís Cláudio Lima, Jaiswal Arun Kumar, Tiwari Sandeep, Carvalho Rodrigo, Benko-Iseppon Ana Maria, Brenig Bertram, Azevedo Vasco, Barh Debmalya, Martins Flaviano S, Aburjaile Flavia
Laboratory of Integrative Bioinformatics, Preventive Veterinary Medicine Department, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
Graduate Program in Bioinformatics, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
Probiotics Antimicrob Proteins. 2025 Apr;17(2):500-515. doi: 10.1007/s12602-023-10170-7. Epub 2023 Oct 7.
Bacteria of the Leuconostoc genus are Gram-positive bacteria that are commonly found in raw milk and persist in fermented dairy products and plant food. Studies have already explored the probiotic potential of L. mesenteroides, but not from a probiogenomic perspective, which aims to explore the molecular features responsible for their phenotypes. In the present work, probiogenomic approaches were applied in strains F-21 and F-22 of L. mesenteroides isolated from human milk to assess their biosafety at the molecular level and to correlate molecular features with their potential probiotic characteristics. The complete genome of strain F-22 is 1.99 Mb and presents one plasmid, while the draft genome of strain F-21 is 1.89 Mb and presents four plasmids. A high percentage of average nucleotide identity among other genomes of L. mesenteroides (≥ 96%) corroborated the previous taxonomic classification of these isolates. Genomic regions that influence the probiotic properties were identified and annotated. Both strains exhibited wide genome plasticity, cell adhesion ability, proteolytic activity, proinflammatory and immunomodulation capacity through interaction with TLR-NF-κB and TLR-MAPK pathway components, and no antimicrobial resistance, denoting their potential to be candidate probiotics. Further, the strains showed bacteriocin production potential and the presence of acid, thermal, osmotic, and bile salt resistance genes, indicating their ability to survive under gastrointestinal stress. Taken together, our results suggest that L. mesenteroides F-21 and F-22 are promising candidates for probiotics in the food and pharmaceutical industries.
明串珠菌属细菌是革兰氏阳性菌，常见于生牛奶中，并在发酵乳制品和植物性食品中存在。已有研究探索了肠系膜明串珠菌的益生菌潜力，但并非从益生菌基因组学角度进行探索，该角度旨在探究其表型背后的分子特征。在本研究中，将益生菌基因组学方法应用于从人乳中分离出的肠系膜明串珠菌F - 21和F - 22菌株，以在分子水平评估其生物安全性，并将分子特征与其潜在的益生菌特性相关联。菌株F - 22的完整基因组为1.99 Mb，有一个质粒，而菌株F - 21的草图基因组为1.89 Mb，有四个质粒。肠系膜明串珠菌其他基因组之间的平均核苷酸同一性百分比很高（≥96%），证实了这些分离株先前的分类学分类。确定并注释了影响益生菌特性的基因组区域。两株菌均表现出广泛的基因组可塑性、细胞黏附能力、蛋白水解活性、通过与TLR - NF - κB和TLR - MAPK信号通路成分相互作用的促炎和免疫调节能力，且无抗菌抗性，表明它们有成为候选益生菌的潜力。此外，这些菌株显示出产生细菌素的潜力以及存在耐酸、耐热、耐渗透压和耐胆盐基因，表明它们在胃肠道应激下的生存能力。综上所述，我们的结果表明肠系膜明串珠菌F - 21和F - 22在食品和制药行业中是有前景的益生菌候选菌株。
