Jeon Hye Hee, Kim Kyung Hyun, Chun Byung Hee, Ryu Byung Hee, Han Nam Soo, Jeon Che Ok
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Department of Fresh Food Research, Division of Research and Technology, Daesang Co., Ltd, Seoul 02586, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2225-2230. doi: 10.1099/ijsem.0.001930. Epub 2017 Jul 3.
The type strains of four subspecies of Leuconostocmesenteroides, L. mesenteroidessubsp. mesenteroides, L. mesenteroidessubsp. cremoris, L. mesenteroidessubsp. dextranicum and L. mesenteroidessubsp. suionicum, and strain DRC1506T, used as a starter culture for commercial kimchi production in Korea, were phylogenetically analyzed on the basis of their complete genome sequences. Although the type strains of the four L. mesenteroides subspecies and strain DRC1506T shared very high 16S rRNA gene sequence similarities (>99.72 %), the results of analysis of average nucleotide identity (ANI), in silico DNA-DNA hybridization (DDH) and core-genome-based relatedness indicated that they could form five different phylogenetic lineages. The type strains of L. mesenteroidessubsp. mesenteroides, L. mesenteroidessubsp. cremoris and L. mesenteroidessubsp. dextranicum and DRC1506T shared higher ANI and in silico DDH values than the thresholds (95-96 % and 70 %, respectively) generally accepted for different species delineation, whereas the type strain of L. mesenteroidessubsp. suionicum (DSM 20241T) shared lower ANI (<94.1 %) and in silico DDH values (<57.0 %) with the other four L. mesenteroides lineage strains, indicating that DSM 20241T couldn be reclassified as representing a different species. Here, we report that DRC1506T represents a novel subspecies within the species Leuconostoc mesenteroides, for which the name Leuconostoc mesenteroidessubsp. jonggajibkimchii subsp. nov. is proposed. The type strain is DRC1506T (=KCCM 43249T=JCM 31787T). In addition, L. mesenteroidessubsp. suionicum is also reclassified as Leuconostoc suionicum. sp. nov. (type strain DSM 20241T=ATCC 9135T=LMG 8159T=NCIMB 6992T).
基于嗜柠檬酸明串珠菌（Leuconostoc mesenteroides）四个亚种嗜柠檬酸明串珠菌肠系膜亚种（L. mesenteroides subsp. mesenteroides）、嗜柠檬酸明串珠菌乳脂亚种（L. mesenteroides subsp. cremoris）、嗜柠檬酸明串珠菌右旋糖酐亚种（L. mesenteroides subsp. dextranicum）和嗜柠檬酸明串珠菌猪源亚种（L. mesenteroides subsp. suionicum）的模式菌株以及韩国商业泡菜生产中用作发酵剂的DRC1506T菌株的全基因组序列进行了系统发育分析。尽管嗜柠檬酸明串珠菌四个亚种的模式菌株与DRC1506T菌株具有非常高的16S rRNA基因序列相似性（>99.72%），但平均核苷酸同一性（ANI）分析、电子DNA-DNA杂交（DDH）分析和基于核心基因组的亲缘关系分析结果表明，它们可形成五个不同的系统发育谱系。嗜柠檬酸明串珠菌肠系膜亚种、嗜柠檬酸明串珠菌乳脂亚种、嗜柠檬酸明串珠菌右旋糖酐亚种的模式菌株以及DRC1506T菌株共享的ANI和电子DDH值高于通常用于不同物种划分的阈值（分别为95 - 96%和70%），而嗜柠檬酸明串珠菌猪源亚种（DSM 20241T）与其他四个嗜柠檬酸明串珠菌谱系菌株共享的ANI（<94.1%）和电子DDH值（<57.0%）较低，这表明DSM 20241T不能重新分类为代表一个不同的物种。在此，我们报告DRC1506T代表嗜柠檬酸明串珠菌物种内的一个新亚种，为此提出名称嗜柠檬酸明串珠菌jonggajibkimchii亚种（Leuconostoc mesenteroides subsp. jonggajibkimchii subsp. nov.）。模式菌株为DRC1506T（=KCCM 43249T=JCM 31787T）。此外，嗜柠檬酸明串珠菌猪源亚种也重新分类为猪源明串珠菌（Leuconostoc suionicum）。新种（模式菌株DSM 20241T=ATCC 9135T=LMG 8159T=NCIMB 6992T）。
