Rocha Beatriz Macedo de Oliveira, Sabino Yasmin Neves Vieira, de Almeida Thaís Costa, Palacio Fabio Bignoto, Rotta Isabela Sguilla, Dias Vanessa Cordeiro, da Silva Vânia Lúcia, Diniz Cláudio Galuppo, Azevedo Vasco Ariston de Carvalho, Brenig Bertram, Soares Siomar de Castro, Paiva Aline Dias, Medeiros Julliane Dutra, Machado Alessandra Barbosa Ferreira
Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil.
Universidade Federal Do Triângulo Mineiro (UFTM), Uberaba, Brazil.
Probiotics Antimicrob Proteins. 2024 Dec 5. doi: 10.1007/s12602-024-10409-x.
Weissella, a genus of lactic acid bacteria, has diverse beneficial attributes including probiotic activity and biotechnological applications. Therefore, the investigation of the Weissella genus has garnered growing interest. In this study, we sequenced the complete genome of Weissella paramesenteroides UFTM 2.6.1 isolated from unpasteurized cow's milk from the Triângulo Mineiro region and performed probiogenomic analyses. Taxonomic characterization confirmed the identity of W. paramesenteroides. The genome comprises 1926 protein-coding genes, mainly related to cell metabolism, information storage and processing, and cellular processes and signaling. Ninety-nine unique genes associated with probiotic functions were identified in the genome of W. paramesenteroides UFTM 2.6.1, including genes involved in stress response, bacterial persistence in the gastrointestinal tract, and biosynthesis of vitamins. In silico analysis of bacteriocin-related genes identified Pediocin, and subsequent in vitro testing confirmed that W. paramesenteroides UFTM 2.6.1 exhibits antimicrobial activity against Listeria spp. Genomic characterization revealed the presence of the replicon pLCK4 and four prophage regions, one of which was intact. Moreover, no CRISPR-Cas array or associated Cas proteins were found, along with an absence of resistance and virulence genes, suggesting a safety aspect of the evaluated strain. Pan-genome analysis unveiled 204 exclusive genes in the genome of W. paramesenteroides UFTM 2.6.1, which includes metabolism and stress-associated genes. In general, the results indicate probiotic potential of W. paramesenteroides UFTM 2.6.1. Further studies are required to ensure the safety and beneficial effects of this bacterium in vivo, aiming for future applications in the food industry and animal and human medicine.
魏斯氏菌属是乳酸菌的一个属，具有多种有益特性，包括益生菌活性和生物技术应用。因此，对魏斯氏菌属的研究越来越受到关注。在本研究中，我们对从米纳斯吉拉斯三角地区未经巴氏杀菌的牛奶中分离出的副干酪魏斯氏菌UFTM 2.6.1的全基因组进行了测序，并进行了益生菌基因组分析。分类学鉴定证实了副干酪魏斯氏菌的身份。该基因组包含1926个蛋白质编码基因，主要与细胞代谢、信息存储与处理以及细胞过程与信号传导有关。在副干酪魏斯氏菌UFTM 2.6.1的基因组中鉴定出99个与益生菌功能相关的独特基因，包括参与应激反应、在胃肠道中细菌存活以及维生素生物合成的基因。对细菌素相关基因的电子分析鉴定出片球菌素，随后的体外测试证实副干酪魏斯氏菌UFTM 2.6.1对李斯特菌属具有抗菌活性。基因组特征显示存在复制子pLCK4和四个前噬菌体区域，其中一个是完整的。此外，未发现CRISPR-Cas阵列或相关的Cas蛋白，也没有抗性和毒力基因，这表明所评估菌株具有安全性。泛基因组分析揭示了副干酪魏斯氏菌UFTM 2.6.1基因组中有204个独特基因，包括代谢和应激相关基因。总体而言，结果表明副干酪魏斯氏菌UFTM 2.6.1具有益生菌潜力。需要进一步研究以确保该细菌在体内的安全性和有益效果，以期未来在食品工业以及动物和人类医学中得到应用。
