Department of Pharmacy, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
BMC Complement Med Ther. 2023 Oct 7;23(1):357. doi: 10.1186/s12906-023-04189-0.
Breast cancer is the most common malignancy in women, and medicinal plants can prevent and play an inhibitory role for cancer. This study aims to evaluate the anticancer effect of colchicum autumnale L. Corm on breast cancer cell models.
In this study, the alkaloid-rich extract was prepared using the percolation method and with methanol/water solvent (70:30). HFF2 normal cell line and MCF-7 breast cancer cell line were cultured in microplates (96 wells). Then cells were treated with concentrations of 62.5 to 2000 ng/ml of extract and concentrations of 62 to 1000 ng/ml of doxorubicin at regular intervals of 48 and 72 h, and the percentage of cell growth inhibition was calculated. Cytotoxicity of drugs was measured by the MTT assay method. IC50 values were calculated by Calcusyn software. Also, the P-value of < 0.05 was considered significant.
Alkaloid-rich extract of Colchicum autumnale plant inhibited breast cancer cell growth (MCF-7). The IC50 parameter showed more cytotoxic effects of Colchicum autumnale plant extract on the MCF-7 cancer cell line than HFF2 normal cell line for 48 and 72 h. In addition, with higher concentrations of the extract, cytotoxicity, and growth inhibitory effect increased significantly and in comparison to the doxorubicin was almost the same as cytotoxic.
This research provides a novel view into the development of new drugs for the treatment of cancer diseases. Colchicum autumnale plant extract had a significant cytotoxic effect like Doxorubicin drug on breast cancer cell line (MCF-7), which can alternatively treat and prevent breast cancer.
乳腺癌是女性最常见的恶性肿瘤，药用植物可以预防和发挥抗癌作用。本研究旨在评估秋水仙碱对乳腺癌细胞模型的抗癌作用。
本研究采用渗漉法和甲醇/水溶剂（70:30）制备富含生物碱的提取物。将 HFF2 正常细胞系和 MCF-7 乳腺癌细胞系培养在微孔板（96 孔）中。然后，用浓度为 62.5 至 2000 ng/ml 的提取物和浓度为 62 至 1000 ng/ml 的阿霉素处理细胞，每隔 48 和 72 h 测量细胞生长抑制率。用 MTT 法测定药物的细胞毒性。用 Calcusyn 软件计算 IC50 值。P 值<0.05 被认为有统计学意义。
秋水仙碱植物的富含生物碱提取物抑制乳腺癌细胞（MCF-7）生长。IC50 参数表明，与 HFF2 正常细胞系相比，秋水仙碱植物提取物对 MCF-7 癌细胞系的细胞毒性作用更强，作用时间为 48 和 72 h。此外，随着提取物浓度的增加，细胞毒性和生长抑制作用显著增加，与阿霉素相比几乎相同。
本研究为开发治疗癌症疾病的新药提供了新的视角。秋水仙碱植物提取物对乳腺癌细胞系（MCF-7）具有与阿霉素药物相当的显著细胞毒性作用，可替代治疗和预防乳腺癌。
