Guangdong Pharmaceutical University, Guangzhou, China.
Beijing Institute of Radiation Medicine, Beijing, China.
Nat Prod Res. 2024 Jan-Feb;38(2):278-286. doi: 10.1080/14786419.2022.2118745. Epub 2022 Sep 5.
A new alkaloid, demethylorychophragine A (), together with 18 known compounds was isolated from the aerial parts of Structure of the new compound was identified by extensive spectroscopic techniques. and were isolated from nature for the first time. Known compounds were identified by comparison with data reported in the literature. All compounds were evaluated for anti-radiation activities against X-ray irradiation in HUVEC cell line. Results showed that , , and showed excellent anti-radiation activity. All compounds were tested for cytotoxic activity against SH-SY5Y and HeLa cell lines. exhibited significant cytotoxicity activity against SH-SY5Y cells, with IC value of 23.86 ± 0.38 µM (p = 0.045). The anti-inflammatory effects of all compounds were assessed by determining their ability to inhibit the production of NO, which was induced by stimulating the RAW264.7 murine macrophage cell line with lipopolysaccharide. markedly decreased NO to a level of 27.34 ± 1.29 µM.
从马先蒿属植物的地上部分分离得到一个新的生物碱 demethylorychophragine A ()，以及 18 个已知化合物。通过广泛的光谱技术鉴定了新化合物的结构。 和 首次从自然界中分离得到。通过与文献报道的数据进行比较，鉴定了已知化合物。所有化合物均在 HUVEC 细胞系中进行了抗辐射活性(X 射线照射)评估。结果表明， 、 、 具有优异的抗辐射活性。所有化合物均对 SH-SY5Y 和 HeLa 细胞系进行了细胞毒性活性测试。 对 SH-SY5Y 细胞表现出显著的细胞毒性活性，IC 值为 23.86 ± 0.38 μM(p = 0.045)。通过测定化合物抑制脂多糖刺激 RAW264.7 鼠巨噬细胞系产生一氧化氮(NO)的能力，评估了所有化合物的抗炎作用。 显著降低 NO 至 27.34 ± 1.29 μM 的水平。
