• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人类大脑成熟过程中细胞类型特异性基因表达动态

Cell type-specific gene expression dynamics during human brain maturation.

作者信息

Steyn Christina, Mishi Ruvimbo, Fillmore Stephanie, Verhoog Matthijs B, More Jessica, Rohlwink Ursula K, Melvill Roger, Butler James, Enslin Johannes M N, Jacobs Muazzam, Sauka-Spengler Tatjana, Greco Maria, Quiñones Sadi, Dulla Chris G, Raimondo Joseph V, Figaji Anthony, Hockman Dorit

机构信息

Division of Cell Biology, Department of Human Biology, University of Cape Town, Cape Town, South Africa.

Neuroscience Institute, University of Cape Town, Cape Town, South Africa.

出版信息

bioRxiv. 2024 May 17:2023.09.29.560114. doi: 10.1101/2023.09.29.560114.

DOI:10.1101/2023.09.29.560114
PMID:37808657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10557738/
Abstract

The human brain undergoes protracted post-natal maturation, guided by dynamic changes in gene expression. Most studies exploring these processes have used bulk tissue analyses, which mask cell type-specific gene expression dynamics. Here, using single nucleus (sn)RNA-seq on temporal lobe tissue, including samples of African ancestry, we build a joint paediatric and adult atlas of 75 cell subtypes, which we verify with spatial transcriptomics. We explore the differences between paediatric and adult cell types, revealing the genes and pathways that change during brain maturation. Our results highlight excitatory neuron subtypes, including the and subtypes, that show elevated expression of genes associated with cognition and synaptic plasticity in paediatric tissue. The new resources we present here improve our understanding of the brain during its development and contribute to global efforts to build an inclusive brain cell map.

摘要

人类大脑在出生后经历长期成熟过程，由基因表达的动态变化引导。大多数探索这些过程的研究都使用了大块组织分析，这掩盖了细胞类型特异性的基因表达动态。在这里，我们对颞叶组织（包括非洲血统样本）进行单核(sn)RNA测序，构建了一个包含75种细胞亚型的儿童和成人联合图谱，并通过空间转录组学进行了验证。我们探索了儿童和成人细胞类型之间的差异，揭示了大脑成熟过程中发生变化的基因和通路。我们的结果突出了兴奋性神经元亚型，包括 和 亚型，这些亚型在儿童组织中显示出与认知和突触可塑性相关基因的表达升高。我们在此展示的新资源增进了我们对大脑发育过程的理解，并有助于全球构建包容性脑细胞图谱的努力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/f9c4f00c1beb/nihpp-2023.09.29.560114v2-f0017.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/838bc8b7b4b3/nihpp-2023.09.29.560114v2-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/f9f9ddb0299e/nihpp-2023.09.29.560114v2-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/163e3672d75a/nihpp-2023.09.29.560114v2-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/631f844784f1/nihpp-2023.09.29.560114v2-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/71d5bb3ee6d7/nihpp-2023.09.29.560114v2-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/af3b8e76348a/nihpp-2023.09.29.560114v2-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/eae930ccc457/nihpp-2023.09.29.560114v2-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/4a7b40fc2792/nihpp-2023.09.29.560114v2-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/011009f650e6/nihpp-2023.09.29.560114v2-f0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/4b3bf63a44a3/nihpp-2023.09.29.560114v2-f0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/27a3df6875b3/nihpp-2023.09.29.560114v2-f0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/54852c9944bd/nihpp-2023.09.29.560114v2-f0012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/498b35815b68/nihpp-2023.09.29.560114v2-f0013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/fa6234895a3e/nihpp-2023.09.29.560114v2-f0014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/8d0ac16f2e8f/nihpp-2023.09.29.560114v2-f0015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/869c99a4e3a4/nihpp-2023.09.29.560114v2-f0016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/f9c4f00c1beb/nihpp-2023.09.29.560114v2-f0017.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/838bc8b7b4b3/nihpp-2023.09.29.560114v2-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/f9f9ddb0299e/nihpp-2023.09.29.560114v2-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/163e3672d75a/nihpp-2023.09.29.560114v2-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/631f844784f1/nihpp-2023.09.29.560114v2-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/71d5bb3ee6d7/nihpp-2023.09.29.560114v2-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/af3b8e76348a/nihpp-2023.09.29.560114v2-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/eae930ccc457/nihpp-2023.09.29.560114v2-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/4a7b40fc2792/nihpp-2023.09.29.560114v2-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/011009f650e6/nihpp-2023.09.29.560114v2-f0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/4b3bf63a44a3/nihpp-2023.09.29.560114v2-f0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/27a3df6875b3/nihpp-2023.09.29.560114v2-f0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/54852c9944bd/nihpp-2023.09.29.560114v2-f0012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/498b35815b68/nihpp-2023.09.29.560114v2-f0013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/fa6234895a3e/nihpp-2023.09.29.560114v2-f0014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/8d0ac16f2e8f/nihpp-2023.09.29.560114v2-f0015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/869c99a4e3a4/nihpp-2023.09.29.560114v2-f0016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f230/11105904/f9c4f00c1beb/nihpp-2023.09.29.560114v2-f0017.jpg

相似文献

1
Cell type-specific gene expression dynamics during human brain maturation.人类大脑成熟过程中细胞类型特异性基因表达动态
bioRxiv. 2024 May 17:2023.09.29.560114. doi: 10.1101/2023.09.29.560114.
2
A temporal cortex cell atlas highlights gene expression dynamics during human brain maturation.颞叶皮质细胞图谱揭示了人类大脑成熟过程中的基因表达动态变化。
Nat Genet. 2024 Dec;56(12):2718-2730. doi: 10.1038/s41588-024-01990-6. Epub 2024 Nov 20.
3
Single-cell, single-nucleus and xenium-based spatial transcriptomics analyses reveal inflammatory activation and altered cell interactions in the hippocampus in mice with temporal lobe epilepsy.单细胞、单核和基于Xenium的空间转录组学分析揭示了颞叶癫痫小鼠海马中的炎症激活和细胞间相互作用改变。
Biomark Res. 2024 Sep 13;12(1):103. doi: 10.1186/s40364-024-00636-3.
4
Discrete placental gene expression signatures accompany diabetic disease classifications during pregnancy.离散的胎盘基因表达特征伴随着孕期糖尿病疾病分类。
Am J Obstet Gynecol. 2025 Mar;232(3):326.e1-326.e15. doi: 10.1016/j.ajog.2024.05.014. Epub 2024 May 17.
5
Single-nucleus and spatial transcriptomics of paediatric ovary: Molecular insights into the dysregulated signalling pathways underlying premature ovarian insufficiency in classic galactosemia.儿科卵巢的单细胞和空间转录组学：经典半乳糖血症中卵巢功能不全失调信号通路的分子研究。
Clin Transl Med. 2024 Oct;14(10):e70043. doi: 10.1002/ctm2.70043.
6
An Integrative Single-cell Transcriptomic Atlas of the Post-natal Mouse Mammary Gland Allows Discovery of New Developmental Trajectories in the Luminal Compartment.产后小鼠乳腺的综合单细胞转录组图谱有助于发现管腔区新的发育轨迹。
J Mammary Gland Biol Neoplasia. 2021 Mar;26(1):29-42. doi: 10.1007/s10911-021-09488-1. Epub 2021 Apr 28.
7
CDSeqR: fast complete deconvolution for gene expression data from bulk tissues.CDSeqR：从批量组织中进行基因表达数据的快速完整去卷积。
BMC Bioinformatics. 2021 May 24;22(1):262. doi: 10.1186/s12859-021-04186-5.
8
Single-nucleus multi-omics of Parkinson's disease reveals a glutamatergic neuronal subtype susceptible to gene dysregulation via alteration of transcriptional networks.帕金森病的单核多组学研究揭示了一种谷氨酸能神经元亚型，易受基因失调的影响，其机制是转录网络的改变。
Acta Neuropathol Commun. 2024 Jul 2;12(1):111. doi: 10.1186/s40478-024-01803-1.
9
Single-nucleus chromatin accessibility and transcriptomic map of breast tissues of women of diverse genetic ancestry.不同遗传血统女性乳腺组织的单核染色质可及性和转录组图谱。
Nat Med. 2024 Dec;30(12):3482-3494. doi: 10.1038/s41591-024-03011-9. Epub 2024 Aug 9.
10
Gene expression patterns of the developing human face at single cell resolution reveal cell type contributions to normal facial variation and disease risk.单细胞分辨率下发育中人类面部的基因表达模式揭示了细胞类型对正常面部变异和疾病风险的影响。
bioRxiv. 2025 Feb 5:2025.01.18.633396. doi: 10.1101/2025.01.18.633396.

本文引用的文献

1
Recent advances in understanding the human host immune response in tuberculous meningitis.结核性脑膜炎中人类宿主免疫反应的研究进展。
Front Immunol. 2024 Jan 9;14:1326651. doi: 10.3389/fimmu.2023.1326651. eCollection 2023.
2
Interindividual variation in human cortical cell type abundance and expression.人类皮质细胞类型丰度和表达的个体间变异性。
Science. 2023 Oct 13;382(6667):eadf2359. doi: 10.1126/science.adf2359.
3
Single-cell analysis of prenatal and postnatal human cortical development.单细胞分析人类产前和产后皮质发育。
Science. 2023 Oct 13;382(6667):eadf0834. doi: 10.1126/science.adf0834.
4
Genes associated with cognitive ability and HAR show overlapping expression patterns in human cortical neuron types.与认知能力和 HAR 相关的基因在人类皮质神经元类型中表现出重叠的表达模式。
Nat Commun. 2023 Jul 13;14(1):4188. doi: 10.1038/s41467-023-39946-9.
5
A post-transcriptional regulatory landscape of aging in the female mouse hippocampus.雌性小鼠海马体衰老的转录后调控图景。
Front Aging Neurosci. 2023 Mar 24;15:1119873. doi: 10.3389/fnagi.2023.1119873. eCollection 2023.
6
Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets.衰老大脑中 mRNA 和蛋白质表达的解偶联揭示了特定基因子集随年龄变化的适应。
Cells. 2023 Feb 14;12(4):615. doi: 10.3390/cells12040615.
7
Brain Data Standards - A method for building data-driven cell-type ontologies.脑数据标准——一种基于数据驱动的细胞类型本体构建方法。
Sci Data. 2023 Jan 24;10(1):50. doi: 10.1038/s41597-022-01886-2.
8
Teneurin paralogues are able to localise synaptic sites driven by the intracellular domain and have the potential to form -heterodimers.Teneurin同源物能够定位于由细胞内结构域驱动的突触位点，并有可能形成异源二聚体。
Front Neurosci. 2022 Nov 3;16:915149. doi: 10.3389/fnins.2022.915149. eCollection 2022.
9
Differential role of neuronal glucose and PFKFB3 in memory formation during development.神经元葡萄糖和 PFKFB3 在发育过程中记忆形成中的差异作用。
Glia. 2022 Nov;70(11):2207-2231. doi: 10.1002/glia.24248. Epub 2022 Aug 2.
10
psupertime: supervised pseudotime analysis for time-series single-cell RNA-seq data.psupertime：用于时间序列单细胞 RNA-seq 数据的有监督伪时间分析。
Bioinformatics. 2022 Jun 24;38(Suppl 1):i290-i298. doi: 10.1093/bioinformatics/btac227.