Matute Daniel R, Cooper Brandon S
Biology Department, University of North Carolina, 250 Bell Tower Drive, Genome Sciences Building, Chapel Hill, NC 27510.
Division of Biological Sciences, University of Montana, 32 Campus Dr., Missoula, MT 59812.
bioRxiv. 2023 Sep 29:2023.09.29.560125. doi: 10.1101/2023.09.29.560125.
Identifying the current geographic range of disease vectors is a critical first step towards determining effective mechanisms for controlling and potentially eradicating them. This is particularly true given that historical vector ranges may expand due to changing climates and human activity. The subgenus contains over 100 species, and among them, and mosquitoes represent the largest concern for public health, spreading dengue, chikungunya, and Zika viruses. While has been observed in the country of Zambia for decades, has not. In 2015 we sampled four urban and two rural areas in Zambia for species. Using DNA barcoding, we confirmed the presence of immature and adult at two rural sites: Siavonga and Livingstone. These genotypes seem most closely related to specimens previously collected in Mozambique based on CO1 sequence from mtDNA. We resampled Siavonga and Livingstone sites in 2019, again observing immature and adult at both sites. Relative frequencies were similar between sites, with the exception of immature life stages, which were higher in Siavonga than in Livingstone in 2019. While frequencies did not vary through time in Livingstone, both immature and adult frequencies increased through time in Siavonga. This report serves to document the presence of in Zambia, which will contribute to the process of determining the potential public health implications of this disease vector in Central Africa.
确定疾病传播媒介当前的地理分布范围是确定控制并可能根除它们的有效机制的关键第一步。鉴于历史上传播媒介的分布范围可能会因气候变化和人类活动而扩大，这一点尤为重要。该亚属包含100多个物种，其中， 和 蚊子对公共卫生构成最大威胁，传播登革热、基孔肯雅热和寨卡病毒。虽然在赞比亚已被观察到数十年，但 尚未被观察到。2015年，我们在赞比亚的四个城市和两个农村地区对 物种进行了采样。使用DNA条形码技术，我们在两个农村地点（锡安加和利文斯通）确认了未成熟和成熟 的存在。基于线粒体DNA的CO1序列，这些基因型似乎与之前在莫桑比克采集的标本关系最为密切。我们在2019年对锡安加和利文斯通地点重新进行了采样，再次在两个地点观察到未成熟和成熟 。除了未成熟阶段外，两个地点之间的相对 频率相似，2019年锡安加的未成熟阶段频率高于利文斯通。虽然利文斯通的 频率没有随时间变化，但锡安加的未成熟和成熟频率均随时间增加。本报告旨在记录 在赞比亚的存在，这将有助于确定这种疾病传播媒介在中非对公共卫生的潜在影响。
