不同分级口腔鳞状细胞癌中分化簇57与增殖细胞核抗原表达的比较分析：一项免疫组织化学研究
Comparative Analysis of Cluster of Differentiation 57 and Proliferating Cell Nuclear Antigen Expression in Different Grades of Oral Squamous Cell Carcinoma: An Immunohistochemical Study.
作者信息
Cs Ani Simila, Joseph T Isaac, Girish K L, T Prasanth, Binu Angelin, Mary Jeslin
机构信息
Oral and Maxillofacial Pathology, Rajas Dental College and Hospital, Kavalkinaru, IND.
Oral and Maxillofacial Pathology, Sree Mookambika Institute of Dental Sciences, Kulasekaram, IND.
出版信息
Cureus. 2023 Sep 6;15(9):e44779. doi: 10.7759/cureus.44779. eCollection 2023 Sep.
BACKGROUND
The immune defense against tumor cells is mainly mediated by the natural killer (NK) cells. Cluster of differentiation 57 (CD57) is a 110-kd glycoprotein, typically expressed by the NK cells, attacks the cancer cells and inhibits tumor development. Proliferating cell nuclear antigen (PCNA) is a 36-kd auxiliary protein for DNA polymerase delta that correlates with cell proliferation and DNA synthesis. It is an essential component of DNA replication, DNA recombination, and DNA repair. The uncoordinated proliferation of PCNA protein characterizes the biological behavior of malignant lesions.
AIM
The aim of the present study is to compare and correlate the expression of CD57 and PCNA in different grades of oral squamous cell carcinoma (OSCC) by immunohistochemistry.
MATERIALS AND METHODS
This retrospective analysis comprises 30 samples of various grades of OSCCs and 10 samples of healthy mucosa. Sections of 4-5 µm thickness were done and stained with monoclonal anti-PCNA and anti-CD57 antibodies. The statistical package for social science (SPSS) version 16.0 software (IBM Corp., Armonk, NY) was used to analyze the data in this study. The expression of CD57 and PCNA was compared and correlated between the groups using analysis of variance (ANOVA) post hoc, Dunnet , and Pearson's correlation coefficient test. For statistical significance, a p-value of 0.05 or less was used.
RESULTS
A significant decrease in CD57 labeling index was seen from well-differentiated squamous cell carcinoma (16.63 ± 2.33) to poorly differentiated squamous cell carcinoma (5.53 ± 1.20) whereas the significant increase in PCNA labeling index was noted from well-differentiated squamous cell carcinoma (45.88 ± 2.20), followed by moderately differentiated and poorly differentiated squamous cell carcinoma (72.77 ± 4.35).
CONCLUSION
The combination of CD57 and PCNA biomarkers appears to be good indicators of the immune status of the patient and the aggressiveness of the lesion.
背景
针对肿瘤细胞的免疫防御主要由自然杀伤（NK）细胞介导。分化簇57（CD57）是一种110千道尔顿的糖蛋白，通常由NK细胞表达，攻击癌细胞并抑制肿瘤发展。增殖细胞核抗原（PCNA）是一种36千道尔顿的DNA聚合酶δ辅助蛋白，与细胞增殖和DNA合成相关。它是DNA复制、DNA重组和DNA修复的重要组成部分。PCNA蛋白的不协调增殖是恶性病变生物学行为的特征。
目的
本研究旨在通过免疫组织化学比较并关联不同分级口腔鳞状细胞癌（OSCC）中CD57和PCNA的表达。
材料与方法
本回顾性分析包括30份不同分级的OSCC样本和10份健康黏膜样本。制作4 - 5微米厚的切片，并用抗PCNA单克隆抗体和抗CD57抗体染色。本研究使用社会科学统计软件包（SPSS）16.0版软件（IBM公司，纽约州阿蒙克）分析数据。使用方差分析（ANOVA）事后检验、Dunnet检验和Pearson相关系数检验比较并关联各组中CD57和PCNA的表达。对于统计学显著性，采用p值小于或等于0.05。
结果
从高分化鳞状细胞癌（16.63±2.33）到低分化鳞状细胞癌（5.53±1.2），CD57标记指数显著降低，而从高分化鳞状细胞癌（45.88±2.2）开始，PCNA标记指数显著增加，随后是中分化和低分化鳞状细胞癌（72.77±4.35）。
结论
CD57和PCNA生物标志物的组合似乎是患者免疫状态和病变侵袭性的良好指标。
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