Kinami Y, Sakata N, Takata M
Gastroenterol Jpn. 1986 Oct;21(5):499-507. doi: 10.1007/BF02774634.
This study was designed to search for a way to inhibit pancreatic fibrosis following acute pancreatitis. Experimental necrotizing pancreatitis was induced by a freezing procedure in the pancreas of male Wistar rats. After the freezing procedure, the rats were divided into 3 groups: nothing addition was done to the control group, while the other 2 groups received daily intraperitoneal administration of antifibrotic substances (colchicine or L-azetidine-2-carboxylic acid (AZC)) for 6 weeks. Pancreatic enzymes in the serum were not markedly influenced by administration of antifibrotic substances, and there were no differences in the ratios of dry to wet weights of the pancreas between groups with and without these drugs. After freezing, the hydroxyproline levels in the pancreas of the control group increased from 1 to 4 weeks and then decreased during the 5th and 6th weeks. All groups receiving colchicine or AZC exhibited a significant decrease in the hydroxyproline levels at 2 to 4 weeks compared with the control group (P less than 0.01). Histological examination also showed the inhibition of pancreatic fibrosis, agreeing with changes in the hydroxyproline levels in groups receiving colchicine or AZC. These results suggest that administration of antifibrotic substances, colchicine and AZC, have the possibility of inhibiting pancreatic fibrosis following acute pancreatitis.
本研究旨在探寻一种抑制急性胰腺炎后胰腺纤维化的方法。通过冷冻法诱导雄性Wistar大鼠胰腺发生实验性坏死性胰腺炎。冷冻术后，将大鼠分为3组：对照组不做任何处理，另外2组每天腹腔注射抗纤维化物质（秋水仙碱或L-氮杂环丁烷-2-羧酸（AZC）），持续6周。血清中的胰腺酶并未受到抗纤维化物质给药的显著影响，使用和未使用这些药物的组之间胰腺干湿重比也没有差异。冷冻后，对照组胰腺中的羟脯氨酸水平在第1至4周升高，然后在第5和6周下降。与对照组相比，所有接受秋水仙碱或AZC的组在第2至4周时羟脯氨酸水平均显著降低（P小于0.01）。组织学检查也显示胰腺纤维化受到抑制，这与接受秋水仙碱或AZC的组中羟脯氨酸水平的变化一致。这些结果表明，抗纤维化物质秋水仙碱和AZC有可能抑制急性胰腺炎后的胰腺纤维化。
