[近红外光疗法可能通过激活PGC-1/Nrf2信号通路并增加海马脑源性神经营养因子（BDNF）的表达来改善大鼠抑郁症诱导的肠道功能障碍]
[Near-infrared light therapy ameliorates depression-induced intestinal dysfunction in rats possibly by activating PGC-1/Nrf2 signaling and increasing hippocampal BDNF expression].
作者信息
Zheng Y, Hou Y, Li F, Shi X, Tao Y, Zhao X, Hu H, Yang L
机构信息
Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.
Basic Medicine Experimental Teaching Center, Xi'an Jiaotong University, Xi'an 710061, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2023 Sep 20;43(9):1591-1598. doi: 10.12122/j.issn.1673-4254.2023.09.17.
OBJECTIVE
To investigate the effect of near-infrared (NIR) light therapy on depression-induced intestinal dysfunction in rats and explore the possible mechanism.
METHODS
Thirty-two male SD rats were randomly divided into control group, model group, low-dose NIR light group and high-dose NIR light group. All the rats except for those in the control group were subjected to chronic restrained stress (CRS) for 4 weeks, and NIR light therapy of the head was administered in the two NIR light groups. The depression- like behaviors, intestinal functions, fecal water content and number of fecal pellets of the rats were evaluated. HE staining was used for detecting histopathological changes in the hippocampus and colon, and hippocampal expressions of BDNF, Nrf2 and PGC-1 were detected with Western blotting.
RESULTS
The rats in the CRS model group showed significantly increased immobility time and visceral sensitivity in the behavioral tests, decreased fecal pellets and fecal water content, and lowered expressions of BDNF, Nrf2, and PGC-1 in the hippocampus (<0.05). Histopathological examination of the CRS rats revealed loosely arranged hippocampal pyramidal cells, obvious neuronal damages, and obvious inflammatory cell infiltration in the colon with irregularly arranged mucosal glands and a high pathological score. High-dose NIR light therapy significantly lowered the immobility time and visceral sensitivity, increased the number of fecal pellets and fecal water content (<0.05), and enhanced hippocampal expressions of BDNF, Nrf2, and PGC-1 (<0.05) of the depressive rats. The rats receiving high-dose NIR light therapy also exhibited close arrangement of the hippocampal pyramidal cells with significantly reduced neuronal damage and colonic inflammatory cell infiltration, neatly arranged mucosal glands, and lowered pathological score.
CONCLUSION
NIR light therapy can significantly improve depression-like behavior and intestinal function in rats possibly by ameliorating oxidative stress the PGC-1/Nrf2 signaling pathway and increasing BDNF level in the hippocampus.
目的
探讨近红外（NIR）光疗对大鼠抑郁诱导的肠道功能障碍的影响，并探索其可能机制。
方法
将32只雄性SD大鼠随机分为对照组、模型组、低剂量NIR光组和高剂量NIR光组。除对照组外，其余大鼠均接受4周的慢性束缚应激（CRS），并对两个NIR光组大鼠进行头部近红外光疗。评估大鼠的抑郁样行为、肠道功能、粪便含水量和粪便颗粒数。采用苏木精-伊红（HE）染色检测海马和结肠的组织病理学变化，并用蛋白质免疫印迹法检测海马中脑源性神经营养因子（BDNF）、核因子E2相关因子2（Nrf2）和过氧化物酶体增殖物激活受体γ共激活因子1（PGC-1）的表达。
结果
CRS模型组大鼠在行为测试中不动时间和内脏敏感性显著增加，粪便颗粒数和粪便含水量减少，海马中BDNF、Nrf2和PGC-1的表达降低（<0.05）。CRS大鼠的组织病理学检查显示海马锥体细胞排列疏松，神经元损伤明显，结肠中有明显的炎症细胞浸润，黏膜腺体排列不规则，病理评分高。高剂量NIR光疗显著降低了抑郁大鼠的不动时间和内脏敏感性，增加了粪便颗粒数和粪便含水量（<0.05），并增强了海马中BDNF、Nrf2和PGC-1的表达（<0.05）。接受高剂量NIR光疗的大鼠海马锥体细胞排列紧密，神经元损伤和结肠炎症细胞浸润显著减少，黏膜腺体排列整齐，病理评分降低。
结论
近红外光疗可能通过改善氧化应激、激活PGC-1/Nrf2信号通路和提高海马中BDNF水平，显著改善大鼠的抑郁样行为和肠道功能。
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