National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Road, Klong Luang, Pathumthani 12120, Thailand.
National Biobank of Thailand (NBT), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Road, Klong Luang, Pathumthani 12120, Thailand.
J Nat Prod. 2023 Oct 27;86(10):2304-2314. doi: 10.1021/acs.jnatprod.3c00457. Epub 2023 Oct 10.
Investigation of cultivated fruiting bodies of led to the isolation of 11 previously unreported lanostane dimers, ganoweberianones C (), D (), E (), F (), G (), and H () and isoganoweberianones A (), B (), D (), G (), and H (). Six new ganodermanontriol derivatives as three pairs of diastereomers (/, /, and /) and five new ganoweberianic acids (-) were also isolated. A method for semisynthesis of lanostane dimers by condensation of natural lanostanes was established, which was utilized in the structure elucidation and NMR data assignments of the undescribed natural lanostane dimers. Ganoweberianone D () and isoganoweberianone D () showed significant antimalarial activity against K1 (multidrug-resistant strain) with IC values of 0.057 and 0.035 μM, respectively, whereas their cytotoxicity to Vero cells was weaker (IC 8.1 and 19 μM, respectively).
对栽培子实体的研究导致了 11 种以前未报道的羊毛甾烷二聚体的分离，分别为 ganoweberianones C()、D()、E()、F()、G()和 H()以及 isoganoweberianones A()、B()、D()、G()和 H()。还分离得到了六种新的灵芝三醇衍生物，为三对对映异构体（/、/和/）和五种新的灵芝酸（-）。建立了通过天然羊毛甾烷缩合半合成羊毛甾烷二聚体的方法，该方法用于未描述的天然羊毛甾烷二聚体的结构阐明和 NMR 数据归属。Ganoweberianone D()和 isoganoweberianone D()对 K1（多药耐药株）表现出显著的抗疟活性，IC 值分别为 0.057 和 0.035 μM，而它们对 Vero 细胞的细胞毒性较弱（IC 8.1 和 19 μM，分别）。
