Xu Jian, Yang Han, Nie Chengdong, Wang Tao, Qin Xiangyu, Yang Jie, Chang Yuanhang, Nie Siming, Fu Yujie
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University Harbin 150040 China.
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University 150040 Harbin China.
RSC Adv. 2023 Oct 9;13(42):29438-29449. doi: 10.1039/d3ra05698h. eCollection 2023 Oct 4.
Lingonberry are underutilised due to the lack of evaluating active compounds in different parts. In this study, the phytochemical profiles, antioxidant and antiproliferative activities of lingonberry's fruits, leaves and stems from different regions of China were compared. Ninety-five bioactive compounds were rapidly identified using a molecular network based on UPLC-Q-Exactive Orbitrap mass spectrometry. The UPLC-QqQ-MS/MS method combined with principal component analysis (PCA) quantified 18 bioactive components in 6 classes. The highest content of arbutin (15 mg/100 g DW) was found in leaves of Huzhong (P6). Ursolic acid and cyanidin-3--galactoside were highest in fruits of Tahe (P4) (4.5 mg/100 g DW and 3.2 mg/100 g DW, respectively). Antioxidant activities determined by DPPH, ABTS and FRAP methods were significantly correlated with total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC). The results indicate that the strongest antioxidant activity and antiproliferative efficacy are observed in the fruits of Tahe (P4) and leaves of Huzhong (P6), respectively. Our results provide valuable insights into lingonberry's comprehensive development and utilization.
由于缺乏对不同部位活性成分的评估，越橘的利用不足。在本研究中，比较了中国不同地区越橘果实、叶片和茎的植物化学特征、抗氧化和抗增殖活性。使用基于超高效液相色谱-四极杆-静电场轨道阱质谱的分子网络快速鉴定了95种生物活性化合物。超高效液相色谱-三重四极杆串联质谱法结合主成分分析（PCA）对6类18种生物活性成分进行了定量。在呼中（P6）的叶片中发现熊果苷含量最高（15 mg/100 g干重）。在塔河（P4）的果实中，熊果酸和花青素-3-半乳糖苷含量最高（分别为4.5 mg/100 g干重和3.2 mg/100 g干重）。通过DPPH、ABTS和FRAP方法测定的抗氧化活性与总酚含量（TPC）、总黄酮含量（TFC）和总花青素含量（TAC）显著相关。结果表明，分别在塔河（P4）的果实和呼中（P6）的叶片中观察到最强的抗氧化活性和抗增殖效果。我们的结果为越橘的综合开发利用提供了有价值的见解。
