Ogura M, Tanaka T, Furihata K, Shimazu A, Otake N
J Antibiot (Tokyo). 1986 Oct;39(10):1443-9. doi: 10.7164/antibiotics.39.1443.
Protoplast regeneration carried out in a carriomycin producing organism, Streptomyces hygroscopicus 358 AV2, lose carriomycin productivity without loss of carriomycin-resistance and the ability of formation of aerial mycelium. Ethidium bromide treatment on the 358 AV2 strain generated a bald mutant that produced carriomycin and a new antibiotic curromycin. In some other media, however, the parent strain produced curromycin, indicating that the ethidium bromide treatment altered the regulation of antibiotic production. Ethidium bromide treatment on a protoplast-regenerated strain derived from the parent strain resulted in derivatives capable of producing carriomycin and curromycin. These strains were unstable and tended to lose the recovered antibiotic productivity easily.
在产生腐霉素的有机体吸水链霉菌358 AV2中进行原生质体再生，该菌株失去了腐霉素生产力，但未丧失对腐霉素的抗性以及气生菌丝体形成能力。用溴化乙锭处理358 AV2菌株产生了一个光秃突变体，该突变体产生腐霉素和一种新抗生素卷曲霉素。然而，在其他一些培养基中，亲本菌株产生卷曲霉素，这表明溴化乙锭处理改变了抗生素产生的调控。用溴化乙锭处理源自亲本菌株的原生质体再生菌株，得到了能够产生腐霉素和卷曲霉素的衍生物。这些菌株不稳定，容易失去恢复的抗生素生产力。
