Lin Xiaojuan, Wang Shuhao, Liu Xiaoling, Qi Yining, Shi Suna, Li Lin
Department of Clinical Laboratory, The Central Hospital of Xingtai, Xingtai, 054000, Hebei, China.
School of Chemistry and Chemical Engineering, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China.
Mikrochim Acta. 2025 Jul 23;192(8):509. doi: 10.1007/s00604-025-07380-x.
Detecting PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) levels is important for assessing lipid metabolism and cardiovascular disease risk, as well as for optimizing treatment plans. Traditional methods such as ELISA and western blotting, while sensitive, are time-consuming and require skilled personnel. Recent advancements in biosensors offer quicker, more sensitive, and cost-effective alternatives, though they face challenges such as complexity and scalability. In this study, we developed a novel sensing method based on a binding-induced DNA walker-triggered TtAgo-based DNA circuit for highly sensitive detection of PCSK9. This method combines the specific recognition ability of the binding-induced DNA walker and the high sensitivity of the TtAgo-based DNA circuit, allowing for precise detection of PCSK9. Our method demonstrated a limit of detection as low as 2.5 fg/mL, significantly outperforming other techniques. Furthermore, the method showed excellent specificity and stability, retaining 93.1% of its initial signal after 15 days. This method allowed accurate detection of PCSK9 in serum samples. These results indicate that the method is a promising tool for PCSK9 detection in clinical settings.
检测前蛋白转化酶枯草溶菌素9型（PCSK9）水平对于评估脂质代谢和心血管疾病风险以及优化治疗方案至关重要。酶联免疫吸附测定（ELISA）和蛋白质印迹法等传统方法虽然灵敏，但耗时且需要技术熟练的人员。生物传感器的最新进展提供了更快、更灵敏且更具成本效益的替代方法，不过它们面临诸如复杂性和可扩展性等挑战。在本研究中，我们基于结合诱导的DNA步行器触发的基于嗜热栖热菌Argonaute（TtAgo）的DNA电路开发了一种新型传感方法，用于高灵敏度检测PCSK9。该方法结合了结合诱导的DNA步行器的特异性识别能力和基于TtAgo的DNA电路的高灵敏度，能够精确检测PCSK9。我们的方法检测限低至2.5 fg/mL，显著优于其他技术。此外，该方法表现出优异的特异性和稳定性，在15天后仍保留其初始信号的93.1%。该方法能够准确检测血清样本中的PCSK9。这些结果表明该方法是临床环境中检测PCSK9的一种有前景的工具。
