Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Biosens Bioelectron. 2022 Oct 15;214:114501. doi: 10.1016/j.bios.2022.114501. Epub 2022 Jun 25.
On-site and real-time clinical monitoring have been progressed dramatically by integrating biosensor science with portable digital electronic technology. Clustered regularly interspaced short palindromic repeats (CRISPR) with association of RNA-guided nucleases (CrRNA-Cas enzymes) have achieved novel CRISPR/Cas biosensing science as a promising revolutionized diagnostic technology for portable and on-site healthcare monitoring and diagnostics. Among several available CRISPR/Cas systems, CRISPR/Cas12a and CRISPR/Cas13a conjugates are utilized broadly in biosensor design, because of their capability to cleave both target and non-target sequences. With the advantages of portability, cost-effectiveness, facile operation, high durability, and reproducibility, CRISPR/Cas-based biosensing techniques are a perfect choice for designing ultra-sensitive point-of-care diagnostic devices with amplified response signals. In the present review, we summarize the advances in the CRISPR/Cas-based biosensors with the focus on healthcare and diagnostic purposes. The cooperation of nanomaterial engineering with CRISPR/Cas biosensors is also represented to attain a promising viewpoint for offering novel user-friendly test kits for announcing ultra-low levels of diverse targets in the future.
通过将生物传感器科学与便携式数字电子技术相结合，现场实时临床监测取得了显著进展。簇状规则间隔短回文重复序列（CRISPR）与 RNA 指导的核酸酶（CrRNA-Cas 酶）的关联实现了新型 CRISPR/Cas 生物传感科学，作为一种用于便携式和现场医疗保健监测和诊断的有前途的革命性诊断技术。在几种可用的 CRISPR/Cas 系统中，CRISPR/Cas12a 和 CRISPR/Cas13a 缀合物广泛用于生物传感器设计，因为它们能够切割靶序列和非靶序列。由于具有便携性、成本效益、操作简便、高耐久性和可重复性等优点，基于 CRISPR/Cas 的生物传感技术是设计具有放大响应信号的超灵敏即时诊断设备的绝佳选择。在本综述中，我们总结了基于 CRISPR/Cas 的生物传感器在医疗保健和诊断方面的进展。还介绍了纳米材料工程与 CRISPR/Cas 生物传感器的合作，以获得有前途的观点，为未来提供新型用户友好的试剂盒，用于宣布各种低水平目标。
