释放因子1基因的定位与互补研究。
Mapping and complementation studies of the gene for release factor 1.
作者信息
Ryden M, Murphy J, Martin R, Isaksson L, Gallant J
出版信息
J Bacteriol. 1986 Dec;168(3):1066-9. doi: 10.1128/jb.168.3.1066-1069.1986.
Abstract
In Escherichia coli the release factor 1 protein (RF1) recognizes and terminates translation at UAG and UAA codons. Using the technique of ColE1 plasmid integration in polA strains, we have mapped the cloned gene for RF1 to 27 min on the E. coli chromosome. This is the same location as that of the uar gene in which temperature-sensitive mutations increase the suppression of UAG and UAA alleles. In this study we proved that the uar mutation lies in the gene for RF1 by complementation of the uar phenotype with plasmids carrying the RF1 gene and by cloning the uar allele onto the RF1 plasmid by means of homologous recombination. In addition, complementation and P1 mapping data suggest that sueB is also a mutation in the same position as the RF1 gene. We propose that the gene for RF1 be named prfA after protein release factor.
摘要
在大肠杆菌中，释放因子1蛋白（RF1）识别UAG和UAA密码子并终止翻译。利用在polA菌株中整合ColE1质粒的技术，我们已将RF1的克隆基因定位到大肠杆菌染色体上27分钟处。这与uar基因的位置相同，在uar基因中，温度敏感突变会增加对UAG和UAA等位基因的抑制作用。在本研究中，我们通过用携带RF1基因的质粒互补uar表型，并通过同源重组将uar等位基因克隆到RF1质粒上，证明了uar突变位于RF1基因中。此外，互补和P1定位数据表明，sueB也是与RF1基因相同位置的突变。我们建议将RF1基因命名为prfA，以表示蛋白质释放因子。
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本文引用的文献
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