嗜热自养甲烷球菌利用甲酸盐产生的甲烷中碳和氢的来源
Source of carbon and hydrogen in methane produced from formate by Methanococcus thermolithotrophicus.
作者信息
Sparling R, Daniels L
出版信息
J Bacteriol. 1986 Dec;168(3):1402-7. doi: 10.1128/jb.168.3.1402-1407.1986.
Abstract
Methanococcus thermolithotrophicus is able to produce methane either from H2-CO2 or from formate. The route of formate entry into the methanogenic pathway was investigated by using 2H2O or [13C]formate and analysis by mass spectrometry. When cells (H2-CO2 or formate grown) were transferred to formate medium in 95% 2H water, the proportion of 2H in methane was 95%. When cells (H2-CO2 or formate grown) were transferred to media containing [13C]formate in the presence of H2-CO2 or He-CO2, the ratio of 13CH4 to 12CH4 increased over time parallel to the ratio of 13CO2 to 12CO2. The cells catalyzed a significant exchange of label between [13C]formate and 13CO2.
摘要
嗜热栖热甲烷球菌能够利用H₂-CO₂或甲酸盐产生甲烷。通过使用重水（²H₂O）或[¹³C]甲酸盐并进行质谱分析，研究了甲酸盐进入产甲烷途径的路线。当细胞（以H₂-CO₂或甲酸盐培养）转移到含95%重水的甲酸盐培养基中时，甲烷中重氢的比例为95%。当细胞（以H₂-CO₂或甲酸盐培养）在H₂-CO₂或He-CO₂存在下转移到含有[¹³C]甲酸盐的培养基中时，¹³CH₄与¹²CH₄的比例随时间增加，与¹³CO₂与¹²CO₂的比例平行。这些细胞催化了[¹³C]甲酸盐与¹³CO₂之间显著的标记交换。
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