嗜热自养甲烷杆菌产生的甲烷中氢的来源。
Origin of hydrogen in methane produced by Methanobacterium thermoautotrophicum.
作者信息
Daniels L, Fulton G, Spencer R W, Orme-Johnson W H
出版信息
J Bacteriol. 1980 Feb;141(2):694-8. doi: 10.1128/jb.141.2.694-698.1980.
Abstract
The production of deuterated methane by Methanobacterium thermoautotrophicum in H2O-D2O mixtures was examined by high-resolution mass spectrometry. The hydrogen in the methane arose solely from water and not from hydrogen gas. Hydrogen gas served only as an electron source in methanogenesis. A whole-cell product isotope discrimination of 1.5 favoring hydrogen over deuterium was observed in methane production in 81 atom% deuterated water. The distribution of deuterated methane species is described by a simple model of the overall reaction.
摘要
利用高分辨率质谱法研究了嗜热自养甲烷杆菌在H₂O-D₂O混合物中生成氘代甲烷的情况。甲烷中的氢仅来自水而非氢气。氢气仅作为甲烷生成过程中的电子源。在81原子%的重水中进行甲烷生成时，观察到全细胞产物同位素对氢的歧视比对氘大1.5。通过整体反应的简单模型描述了氘代甲烷物种的分布。
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