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嗜热自养甲烷杆菌产生的甲烷中氢的来源。

Origin of hydrogen in methane produced by Methanobacterium thermoautotrophicum.

作者信息

Daniels L, Fulton G, Spencer R W, Orme-Johnson W H

出版信息

J Bacteriol. 1980 Feb;141(2):694-8. doi: 10.1128/jb.141.2.694-698.1980.

DOI:10.1128/jb.141.2.694-698.1980
PMID:7364716
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC293677/
Abstract

The production of deuterated methane by Methanobacterium thermoautotrophicum in H2O-D2O mixtures was examined by high-resolution mass spectrometry. The hydrogen in the methane arose solely from water and not from hydrogen gas. Hydrogen gas served only as an electron source in methanogenesis. A whole-cell product isotope discrimination of 1.5 favoring hydrogen over deuterium was observed in methane production in 81 atom% deuterated water. The distribution of deuterated methane species is described by a simple model of the overall reaction.

摘要

利用高分辨率质谱法研究了嗜热自养甲烷杆菌在H₂O-D₂O混合物中生成氘代甲烷的情况。甲烷中的氢仅来自水而非氢气。氢气仅作为甲烷生成过程中的电子源。在81原子%的重水中进行甲烷生成时，观察到全细胞产物同位素对氢的歧视比对氘大1.5。通过整体反应的简单模型描述了氘代甲烷物种的分布。

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本文引用的文献

1
The methane fermentations of acetate and methanol.乙酸盐和甲醇的甲烷发酵
J Bacteriol. 1957 Jun;73(6):736-42. doi: 10.1128/jb.73.6.736-742.1957.
2
Studies on the methane fermentation. XII. The pathway of hydrogen in the acetate fermentation.甲烷发酵研究。十二。乙酸发酵中氢的途径。
J Bacteriol. 1956 Jun;71(6):644-8. doi: 10.1128/jb.71.6.644-648.1956.
3
Mass spectrometry studies of substituted methanes formed from deutero- and fluoromethylcobalamins.由氘代甲基钴胺素和氟甲基钴胺素形成的取代甲烷的质谱研究。
Biochim Biophys Acta. 1972 Jul 19;273(2):265-74. doi: 10.1016/0304-4165(72)90216-4.
4
Methanobacterium thermoautotrophicus sp. n., an anaerobic, autotrophic, extreme thermophile.嗜热自养甲烷杆菌新种，一种厌氧、自养的极端嗜热菌。
J Bacteriol. 1972 Feb;109(2):707-15. doi: 10.1128/jb.109.2.707-713.1972.
5
A new coenzyme of methyl transfer, coenzyme M.一种新的甲基转移辅酶，辅酶M。
Biochemistry. 1971 Jun 8;10(12):2317-24. doi: 10.1021/bi00788a022.
6
Structure and methylation of coenzyme M(HSCH2CH2SO3).辅酶M（HSCH2CH2SO3）的结构与甲基化
J Biol Chem. 1974 Aug 10;249(15):4879-85.
7
Oxidoreductases involved in cell carbon synthesis of Methanobacterium thermoautotrophicum.参与嗜热自养甲烷杆菌细胞碳合成的氧化还原酶。
J Bacteriol. 1977 Nov;132(2):604-13. doi: 10.1128/jb.132.2.604-613.1977.
8
Energy conservation in chemotrophic anaerobic bacteria.化能营养型厌氧细菌中的能量守恒
Bacteriol Rev. 1977 Mar;41(1):100-80. doi: 10.1128/br.41.1.100-180.1977.
9
New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere.产甲烷菌培养的新方法：在加压气氛中嗜瘤胃产甲烷菌依赖2-巯基乙烷磺酸（HS-CoM）生长。
Appl Environ Microbiol. 1976 Dec;32(6):781-91. doi: 10.1128/aem.32.6.781-791.1976.
10
Proposed structure for coenzyme F420 from Methanobacterium.来自甲烷杆菌的辅酶F420的推测结构。
Biochemistry. 1978 Oct 31;17(22):4583-93. doi: 10.1021/bi00615a002.