鸡肝中中性鞘脂类和神经节苷脂的特性分析

Characterization of neutral sphingolipids and gangliosides from chicken liver.

作者信息

Shiraishi T, Uda Y

出版信息

J Biochem. 1986 Sep;100(3):553-61. doi: 10.1093/oxfordjournals.jbchem.a121746.

DOI:10.1093/oxfordjournals.jbchem.a121746

PMID:3782065

Abstract

The neutral sphingolipids and gangliosides were isolated from 62- and 63-day-old chicken livers and characterized. The total concentration of neutral sphingolipids was 59 nmol/g of liver, and that of gangliosides was 330 nmol/g of liver. The major neutral sphingolipids were free ceramide, galactosylceramide, glucosylceramide, lactosylceramide, galabiosylceramide, and Forssman glycolipid. Galactosylceramide was the most abundant and free ceramide was the second most abundant. The major gangliosides were sialosylgalactosylceramide (GM4) and sialosyllactosylceramide (GM3), each of which contained only N-acetylneuraminic acid as a sialic acid. Sphingosine (d18:1) was a major long-chain base in all the sphingolipids. Considerable amounts of 2-hydroxy fatty acids were present in free ceramide, galactosylceramide, and GM4.

摘要

从62日龄和63日龄鸡肝脏中分离并鉴定了中性鞘脂和神经节苷脂。中性鞘脂的总浓度为59 nmol/g肝脏，神经节苷脂的总浓度为330 nmol/g肝脏。主要的中性鞘脂为游离神经酰胺、半乳糖基神经酰胺、葡萄糖基神经酰胺、乳糖基神经酰胺、二半乳糖基神经酰胺和福斯曼糖脂。半乳糖基神经酰胺含量最高，游离神经酰胺次之。主要的神经节苷脂为唾液酸基半乳糖基神经酰胺（GM4）和唾液酸基乳糖基神经酰胺（GM3），它们均仅含有N-乙酰神经氨酸作为唾液酸。鞘氨醇（d18:1）是所有鞘脂中的主要长链碱。游离神经酰胺、半乳糖基神经酰胺和GM4中存在大量的2-羟基脂肪酸。