肾上腺中神经节苷脂和中性糖脂的种间比较。

An interspecies comparison of gangliosides and neutral glycolipids in adrenal glands.

作者信息

Sekine M, Ariga T, Miyatake T, Kase R, Suzuki A, Yamakawa T

出版信息

J Biochem. 1985 Apr;97(4):1219-27. doi: 10.1093/oxfordjournals.jbchem.a135167.

DOI:10.1093/oxfordjournals.jbchem.a135167

Abstract

Gangliosides and neutral glycolipids of adrenal glands of mouse, rat, guinea pig, rabbit, cat, pig, cow, monkey, and chicken were analyzed by thin layer chromatography (TLC). The major gangliosides common to all species had lactosylceramide in their core structure. GM3 containing N-acetylneuraminic acid (NeuAc) was the major ganglioside in rat, guinea pig, rabbit, and cat, whereas GM3 containing N-glycolylneuraminic acid (NeuGc) was the major one in mouse, cow, and monkey. GD3 was also detected in all species except mouse and GD3(NeuAc)2 was the major in pig adrenal gland. GM4(NeuAc) was detected in the adrenal glands of guinea pig and chicken but not in those of the other species. In the neutral glycolipid fractions, galactosylceramide, glucosylceramide, lactosylceramide, globotriaosylceramide and globotetraosylceramide were detected and the proportions of these glycolipids varied among the species. Guinea pig and chicken adrenal glands contained large amounts of galactosylceramide, this being consistent with the presence of GM4 in these two species. Globopentaosylceramide was detected in mouse, guinea pig, cat, and chicken by the TLC-immunostaining procedure.

摘要

采用薄层色谱法（TLC）分析了小鼠、大鼠、豚鼠、兔子、猫、猪、牛、猴子和鸡肾上腺中的神经节苷脂和中性糖脂。所有物种共有的主要神经节苷脂在其核心结构中含有乳糖神经酰胺。含有N - 乙酰神经氨酸（NeuAc）的GM3是大鼠、豚鼠、兔子和猫中的主要神经节苷脂，而含有N - 糖基神经氨酸（NeuGc）的GM3是小鼠、牛和猴子中的主要神经节苷脂。除小鼠外，在所有物种中均检测到GD3，且GD3（NeuAc）2是猪肾上腺中的主要成分。在豚鼠和鸡的肾上腺中检测到GM4（NeuAc），但在其他物种中未检测到。在中性糖脂组分中，检测到半乳糖神经酰胺、葡萄糖神经酰胺、乳糖神经酰胺、球三糖神经酰胺和球四糖神经酰胺，且这些糖脂的比例在不同物种间有所变化。豚鼠和鸡的肾上腺含有大量半乳糖神经酰胺，这与这两个物种中存在GM4一致。通过TLC免疫染色法在小鼠、豚鼠、猫和鸡中检测到球五糖神经酰胺。