Department of Life Sciences, Chalmers University of Technology, Gothenburg, Sweden.
Moleculent, Stockholm, Sweden.
Methods Mol Biol. 2024;2694:175-202. doi: 10.1007/978-1-0716-3377-9_9.
Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.
纳米受限条件下的 DNA 拉伸允许进行几项重要的研究。在单个 DNA 分子水平上可以可视化 DNA 的遗传内容,并且可以探索受限 DNA 的聚合物物理以及 DNA/蛋白质和其他 DNA/DNA 结合分子相互作用。本章介绍了制造纳米结构、进行实验和分析数据的基本步骤。