Quality and Authentication Unit, Agricultural Product Knowledge and Valorisation Department, Walloon Agricultural Research Centre (CRA-W), Chaussée de Namur 24, 5030 Gembloux, Belgium.
J Agric Food Chem. 2023 Oct 25;71(42):15774-15784. doi: 10.1021/acs.jafc.3c03253. Epub 2023 Oct 12.
The circular economy is one of the main building blocks of the European Green Deal. In this context, the use of former foodstuffs containing ruminant gelatin was recently authorized in nonruminant feed. This minor modification makes it more challenging, if not impossible, to interpret the analytical results of the official control for animal proteins. The presence of ruminant DNA from authorized byproducts (i.e., milk and/or gelatin) may hide the use of prohibited byproducts. The objective of this work was to evaluate the use of sedimentation to increase the sensitivity and specificity of bovine-processed animal proteins (PAPs) detection by mass spectrometry-based proteomics. Both approaches (standard versus optimized method) were evaluated by UHPLC-MS/MS on various animal feeds and samples from an interlaboratory study. The optimized method was able to achieve the adulteration level below the level of 0.1% PAPs required by the European Commission. This approach presents a simple and economical solution to improve the method without the need for new equipment or expertise since it is already in place in the control laboratories.
循环经济是欧洲绿色协议的主要组成部分之一。在此背景下，最近允许在非反刍动物饲料中使用含有反刍动物明胶的前食用物质。如果不是不可能的话，这种微小的修改使得解释动物蛋白官方控制的分析结果变得更加具有挑战性。授权副产品（即牛奶和/或明胶）中的反刍动物 DNA 的存在可能会掩盖禁止使用的副产品。本工作的目的是评估使用沉淀来提高基于质谱的蛋白质组学检测牛加工动物蛋白 (PAP) 的灵敏度和特异性。两种方法（标准方法与优化方法）均通过 UHPLC-MS/MS 在各种动物饲料和实验室间研究的样品上进行了评估。优化后的方法能够达到欧盟委员会要求的 PAPs 低于 0.1%的掺杂水平。该方法提供了一种简单经济的解决方案，无需新设备或专业知识即可改进方法，因为该方法已经在控制实验室中得到应用。
