Deeley J O, Crum L A, Coakley W T
Biochim Biophys Acta. 1979 Jun 13;554(1):90-101. doi: 10.1016/0005-2736(79)90009-9.
Human erythrocytes have been heated and stressed in a novel and controlled manner using rectangular microcapillaries. Heated cells attached to the capillary wall were stressed by liquid flow. Under particular conditions of stress, temperature and incubation time the body of the cell could be pulled in the flow, retaining a connection with the glass by means of a narrow process or tether. The tethers appear as: regularly beaded, irregularly beaded or without beads depending upon the incubation conditions. We have outlined the incubation regimes necessary to achieve these different responses in the temperature range 48--55 degrees C. The cells become less deformable as the incubation is continued beyond an optimum time. The behaviour of the tether is compared with that of a viscoelastic liquid. Circular dichroism studies of ghost membranes show that the denaturation of membrane proteins is partially reversible when incubation times are similar to those required to bring about a loss of deformability.
使用矩形微毛细管以一种新颖且可控的方式对人体红细胞进行加热和施加应力。附着在毛细管壁上的受热细胞受到液流的应力作用。在特定的应力、温度和孵育时间条件下，细胞体可能会被液流拉动，通过一个狭窄的突起或系链与玻璃保持连接。根据孵育条件的不同，系链呈现出：规则串珠状、不规则串珠状或无串珠状。我们已经概述了在48 - 55摄氏度温度范围内实现这些不同反应所需的孵育方案。随着孵育时间超过最佳时间继续进行，细胞的可变形性会降低。将系链的行为与粘弹性液体的行为进行了比较。对空壳膜的圆二色性研究表明，当孵育时间与导致可变形性丧失所需的时间相似时，膜蛋白的变性部分是可逆的。
