Sung K L, Chien S
Institute for Biomedical Engineering, University of California, San Diego, La Jolla 92093-0412.
Chin J Physiol. 1992;35(2):81-94.
The effect of variation in temperature on the rheological properties of human red blood cells was studied by determining the cell deformation in response to aspiration pressure applied via a micropipette. The time history of the deformation was analyzed by the use of a Voigt viscoelastic model consisting of an elastic element (E) in parallel with a viscous element (eta). Viscosity values were obtained during the initial rapid deformation phase (phase I), the later slow deformation phase (phase II), and the recovery phase. With a rise in temperature from 0 to 45 degrees C, both E and eta values decreased. A thermotropic transition temperature was found at approximately 21 degrees C for phase I viscosity and at approximately 9 degrees C for viscosity in the recovery phase, but not for phase II viscosity. These responses to temperature variations provide insights into the viscoelastic properties of the erythrocyte membrane.
通过测定人红细胞在通过微量移液器施加的抽吸压力作用下的细胞变形，研究了温度变化对人红细胞流变学特性的影响。利用由一个弹性元件（E）与一个粘性元件（η）并联组成的Voigt粘弹性模型分析变形的时间历程。在初始快速变形阶段（阶段I）、后期缓慢变形阶段（阶段II）和恢复阶段获得粘度值。随着温度从0℃升高到45℃，E值和η值均降低。在阶段I粘度约21℃和恢复阶段粘度约9℃时发现了一个热致转变温度，但阶段II粘度未发现。这些对温度变化的响应为红细胞膜的粘弹性特性提供了见解。
