Castells M C, Pascual C, Esteban M M, Ojeda J A
J Allergy Clin Immunol. 1986 Dec;78(6):1110-4. doi: 10.1016/0091-6749(86)90258-7.
Allergy to potato is uncommon, and even more uncommon is allergy to potato pollen. The occurrence of both phenomena in the same patient made it possible to study cross-reactivity patterns of potato antigens. An 11-year-old girl, exclusively breast-fed for her first 4 months, developed anaphylactic symptoms after ingestion of potato at 5 months of age when she was fed potato for the first time. Subsequently, she developed urticaria, angioedema, and respiratory and systemic symptoms on contact with potatoes, ingestion of potatoes, and exposure to cooking potatoes or potato pollen. Three allergenic extracts from potato pulp, peel, and pollen were prepared. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and isoelectrofocusing of the three extracts were performed. IgE-mediated allergy to these extracts was demonstrated by means of immediate skin test reactivity, positive passive transfer, RAST, RAST inhibition, and leukocyte histamine release. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the pulp extract followed by electroblotting and autoradiography demonstrated specific IgE antibodies directed against several proteins ranging from 14,000 to 40,000 daltons.
对马铃薯过敏并不常见，而对马铃薯花粉过敏则更为罕见。同一患者出现这两种现象使得研究马铃薯抗原的交叉反应模式成为可能。一名11岁女孩，头4个月完全母乳喂养，5个月大首次食用马铃薯时出现过敏症状。随后，她在接触马铃薯、食用马铃薯以及接触烹饪马铃薯或马铃薯花粉时出现荨麻疹、血管性水肿、呼吸道和全身症状。制备了来自马铃薯果肉、外皮和花粉的三种变应原提取物。对这三种提取物进行了十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳和等电聚焦。通过即时皮肤试验反应性、阳性被动转移、放射性变应原吸附试验（RAST）、RAST抑制试验和白细胞组胺释放试验证实了对这些提取物的IgE介导的过敏反应。对果肉提取物进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，随后进行电印迹和放射自显影，结果显示针对几种分子量在14,000至40,000道尔顿之间的蛋白质的特异性IgE抗体。
