Gray M A, Simam P, Smith G R
J Hyg (Lond). 1986 Oct;97(2):305-15. doi: 10.1017/s0022172400065402.
In two trials the efficacy of inactivated vaccines against contagious bovine pleuropneumonia was tested by exposing vaccinated cattle to droplet infection provided by close contact with experimentally infected 'donors'. Complete protection was given by an extreme form of vaccination in which a heavy suspension of killed Mycoplasma mycoides subsp. mycoides emulsified with Freund's complete adjuvant was given in two large doses. 'Mouse-protective antibody' (MPA) was also produced, i.e. serum transferred to mice 2-4 h before intraperitoneal challenge prevented the development of mycoplasmaemia. However, the study did not answer the question 'Is MPA protective for cattle?'. No protection was given by a milder form of vaccination in which a lighter suspension of killed mycoplasmas emulsified with Freund's incomplete adjuvant was given in a comparatively small dose on a single occasion.
在两项试验中，通过让接种疫苗的牛与实验感染的“供体”密切接触，使其受到飞沫感染，来测试灭活疫苗对牛传染性胸膜肺炎的疗效。一种极端形式的疫苗接种提供了完全保护，即使用弗氏完全佐剂乳化的大量灭活丝状支原体丝状亚种悬液，分两剂大剂量接种。还产生了“小鼠保护抗体”（MPA），即在腹腔注射攻击前2 - 4小时将血清转移到小鼠体内可防止支原体血症的发生。然而，该研究并未回答“MPA对牛有保护作用吗？”这一问题。单次给予相对小剂量的、用弗氏不完全佐剂乳化的较轻的灭活支原体悬液这种较温和的疫苗接种形式，未提供保护。
