Baumgarten H
J Immunol Methods. 1986 Nov 20;94(1-2):91-8. doi: 10.1016/0022-1759(86)90219-x.
A cell ELISA for human leukocytes has been established and standardized. The assay was based on the use of air-dried and methanol-fixed cells which were attached to microplate wells. Cellular antigens were measured through the subsequent binding of monoclonal antibody (MoAb), biotinylated sheep Ig against mouse IgG and streptavidin-peroxidase conjugate. The test was standardized by measuring both the specific antigen and the amount of cellular protein in each single sample and was calibrated either with intact cells or isolated plasma membranes prepared from the cells under study. The detection of the pan T-lymphocyte-specific 3A1 antigen (CD7, P40) in fewer than 5000 cells or less than 50 ng membrane protein was possible.
一种用于人白细胞的细胞酶联免疫吸附测定法已经建立并标准化。该测定法基于使用附着在微孔板孔上的风干并经甲醇固定的细胞。通过单克隆抗体（MoAb）、抗小鼠IgG的生物素化绵羊Ig和链霉亲和素-过氧化物酶偶联物的后续结合来测量细胞抗原。通过测量每个单个样品中的特异性抗原和细胞蛋白量对该测试进行标准化，并用完整细胞或从所研究细胞制备的分离质膜进行校准。能够在少于5000个细胞或少于50 ng膜蛋白中检测全T淋巴细胞特异性3A1抗原（CD7，P40）。
