高热抑制细胞功能并诱导肾细胞癌的免疫原性细胞死亡。
Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma.
机构信息
Department of Urology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, No. 127, Dong Ming Road, Zhengzhou, 450000, China.
Department of Urology, Qingdao Central Hospital, No. 127, Si Liu Nan Road, Qingdao, 266042, China.
出版信息
BMC Cancer. 2023 Oct 12;23(1):972. doi: 10.1186/s12885-023-11106-8.
BACKGROUND
In recent years, hyperthermia has been widely applied as a novel strategy for cancer treatment due to its multiple antitumour effects. In particular, the potential influences of hyperthermia on the tumour immune microenvironment may improve the efficacy of immunotherapies. However, the effect of hyperthermia on renal cell carcinoma (RCC) has not been well characterized until now.
METHODS
In the present study, we primarily evaluated the effects of hyperthermia on cellular function via cellular proliferation, migration, invasion and apoptosis assays. In addition, the influence of hyperthermia on the immunogenicity of RCC cells was analysed using flow cytometry analysis, enzyme-linked immunosorbent assays, and immunofluorescent (IF) staining.
RESULTS
Our results demonstrate that hyperthermia significantly inhibits RCC cell proliferation, migration, and invasion and promotes cell apoptosis. In addition, we verified that hyperthermia improves the immunogenicity of RCC cells by inducing immunogenic cell death.
CONCLUSION
Our findings suggest that hyperthermia is a promising therapeutic strategy for RCC.
背景
近年来，由于其多种抗肿瘤作用，热疗已被广泛应用于癌症治疗的新策略中。特别是，热疗对肿瘤免疫微环境的潜在影响可能会提高免疫疗法的疗效。然而，直到现在，热疗对肾细胞癌（RCC）的影响还没有得到很好的描述。
方法
在本研究中，我们主要通过细胞增殖、迁移、侵袭和凋亡实验评估了热疗对细胞功能的影响。此外，我们还通过流式细胞术分析、酶联免疫吸附试验和免疫荧光（IF）染色分析了热疗对 RCC 细胞免疫原性的影响。
结果
我们的结果表明，热疗可显著抑制 RCC 细胞的增殖、迁移和侵袭，促进细胞凋亡。此外，我们验证了热疗通过诱导免疫原性细胞死亡来提高 RCC 细胞的免疫原性。
结论
我们的研究结果表明，热疗是治疗 RCC 的一种很有前途的治疗策略。
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