Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
Institute of Clinical Medicine Research, College of Medicine, Catholic University of Korea, Seoul 06591, Republic of Korea.
Cells. 2023 Sep 26;12(19):2360. doi: 10.3390/cells12192360.
This study investigated the influence of hypoxic culture conditions on human nasal inferior turbinate-derived stem cells (hNTSCs), a subtype of mesenchymal stem cells (MSCs). It aimed to discern how hypoxia affected hNTSC characteristics, proliferation, and differentiation potential compared to hNTSCs cultured under normal oxygen levels. After obtaining hNTSCs from five patients, the samples were divided into hypoxic and normoxic groups. The investigation utilized fluorescence-activated cell sorting (FACS) for surface marker analysis, cell counting kit-8 assays for proliferation assessment, and multiplex immunoassays for cytokine secretion study. Differentiation potential-osteogenic, chondrogenic, and adipogenic-was evaluated via histological examination and gene expression analysis. Results indicated that hNTSCs under hypoxic conditions preserved their characteristic MSC phenotype, as confirmed by FACS analysis demonstrating the absence of hematopoietic markers and presence of MSC markers. Proliferation of hNTSCs remained unaffected by hypoxia. Cytokine expression showed similarity between hypoxic and normoxic groups throughout cultivation. Nevertheless, hypoxic conditions reduced the osteogenic and promoted adipogenic differentiation potential, while chondrogenic differentiation was relatively unchanged. These insights contribute to understanding hNTSC behavior in hypoxic environments, advancing the development of protocols for stem cell therapies and tissue engineering.
本研究旨在探讨低氧培养条件对人鼻下鼻甲衍生干细胞（hNTSCs）的影响，hNTSCs 是间充质干细胞（MSCs）的一种亚型。研究目的是比较 hNTSCs 在常氧和低氧条件下的特性、增殖和分化潜能。从五名患者中获得 hNTSCs 后，将样本分为低氧和常氧组。通过流式细胞术（FACS）进行表面标志物分析、细胞计数试剂盒-8 检测用于增殖评估，以及多重免疫分析用于细胞因子分泌研究。通过组织学检查和基因表达分析评估分化潜能-成骨、成软骨和成脂。结果表明，低氧条件下的 hNTSCs 保持其 MSC 表型特征，FACS 分析证实其缺乏造血标志物，存在 MSC 标志物。低氧对 hNTSCs 的增殖没有影响。细胞因子表达在整个培养过程中在低氧和常氧组之间相似。然而，低氧条件下降低了成骨和促进了成脂分化潜能，而成软骨分化相对不变。这些研究结果有助于了解 hNTSCs 在低氧环境下的行为，推进干细胞治疗和组织工程的方案开发。
