Subchronic toxicity of iron-selenium nanoparticles on oxidative stress response, histopathological, and nuclear damage in amphibian larvae Rana saharica.

In this work, we evaluated the subchronic toxicity of FeSe nanoparticles (NPs) in tadpoles of Rana saharica. Tadpoles were exposed for 1-3 weeks to FeSe NPs at 5 mg/L and 100 mg/L rates. Parameters of oxidative stress were measured in whole larvae, and the micronucleus test was performed on circulating blood erythrocytes. We noted a disturbance of the detoxification systems. Enzymatic and non-enzymatic data showed that exposure to FeSe NPs involved a highly significant depletion of GSH, a significant increase in GST activity, and a lipid peroxidation associated with a highly significant increase in MDA. We also noted a neurotoxic effect characterized by a significant inhibition of AChE activity. A micronucleus test showed concentration-dependent DNA damage. This research reveals that these trace elements, in their nanoform, can cause significant neurotoxicity, histopathologic degeneration, cellular and metabolic activity, and genotoxic consequences in Rana larvae.