School of Information Science and Technology, Fudan University, 2005th Songhu Road, Shanghai 200438, China.
Academy for Engineering and Technology, Fudan University, 220th Handan Road, Shanghai 200433, China.
J Photochem Photobiol B. 2023 Nov;248:112799. doi: 10.1016/j.jphotobiol.2023.112799. Epub 2023 Oct 6.
Antimicrobial blue light (ABL) therapy is one of the novel non-antibiotic approaches and recent studies showed the potential of pulsed ABL.
Comparing photoinactivation effect of continuous wave (CW) and pulsed blue light and investigating the impact of varying light parameters.
E. coli cells in planktonic were treated with CW and pulsed light (405 nm and 450 nm) at 60 mW/cm, and the samples were taken to assess survival, reactive oxygen species (ROS) level, damage of cell membrane and metabolic activity. Further, a ROS scavenger was used to find the role of ROS played in ABL therapy.
E. coli was more sensitive to 405 nm light and the photoinactivation was dose-dependent. Pulsed 405 nm light showed the better antimicrobial effect on E. coli and caused increasing damage of cell membrane. It might be attributed to the ROS production in bacteria.
Pulsed light has a potential of improving the efficacy of ABL therapy and is worth to be explored deeply further.
抗菌蓝光（ABL）疗法是一种新型的非抗生素方法，最近的研究表明脉冲 ABL 有潜力。
比较连续波（CW）和脉冲蓝光的光灭活效果，并研究不同光参数的影响。
用 CW 和脉冲光（405nm 和 450nm）在 60mW/cm 下处理浮游状态的大肠杆菌细胞，并取样评估存活、活性氧（ROS）水平、细胞膜损伤和代谢活性。此外，还使用了一种 ROS 清除剂来确定 ROS 在 ABL 治疗中的作用。
大肠杆菌对 405nm 光更敏感，光灭活具有剂量依赖性。脉冲 405nm 光对大肠杆菌具有更好的抗菌效果，并导致细胞膜损伤增加。这可能归因于细菌中 ROS 的产生。
脉冲光有可能提高 ABL 治疗的疗效，值得进一步深入研究。
