Institute of Physical Culture Sciences, University of Szczecin, 17C Narutowicza St., 70-240, Szczecin, Poland.
Department of Pathology, Pomeranian Medical University in Szczecin, 1 Unii Lubelskiej St., 71-242, Szczecin, Poland.
Sci Rep. 2023 Oct 13;13(1):17431. doi: 10.1038/s41598-023-44483-y.
Currently, there are numerous methods that can be used to neutralize pathogens (i.e., devices, tools, or protective clothing), but the sterilizing agent must be selected so that it does not damage or change the properties of the material to which it is applied. Dry sterilization with hydrogen peroxide gas (VHP) in combination with UV-C radiation is well described and effective method of sterilization. This paper presents the design, construction, and analysis of a novel model of sterilization device. Verification of the sterilization process was performed, using classical microbiological methods and flow cytometry, on samples containing Geobacillus stearothermophilus spores, Bacillus subtilis spores, Escherichia coli, and Candida albicans. Flow cytometry results were in line with the standardized microbiological tests and confirmed the effectiveness of the sterilization process. It was also determined that mobile sterilization stations represent a valuable solution when dedicated to public institutions and businesses in the tourism sector, sports & fitness industry, or other types of services, e.g., cosmetic services. A key feature of this solution is the ability to adapt the device within specific constraints to the user's needs.
目前，有许多方法可以用来中和病原体（即设备、工具或防护服），但必须选择消毒剂，使其不会损坏或改变所应用材料的性质。过氧化氢气体（VHP）与 UV-C 辐射相结合的干法灭菌是一种经过充分描述且有效的灭菌方法。本文介绍了一种新型灭菌设备的设计、构建和分析。使用经典的微生物学方法和流式细胞术，对含有嗜热脂肪地芽孢杆菌孢子、枯草芽孢杆菌孢子、大肠杆菌和白色念珠菌的样本进行了灭菌过程的验证。流式细胞术的结果与标准化的微生物学测试一致，证实了灭菌过程的有效性。还确定了移动灭菌站在面向旅游部门、体育和健身行业或其他类型的服务（例如美容服务）的公共机构和企业具有重要的应用价值。该解决方案的一个关键特点是能够根据用户的需求，在特定限制内调整设备。
