Department of Systems Biotechnology, and Institute of Microbiomics, Chung-Ang University, Anseong, 17546, Republic of Korea.
Environmental Diseases Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.
Sci Rep. 2023 Oct 13;13(1):17412. doi: 10.1038/s41598-023-44612-7.
Prodigiosin, a red pigment produced by Hahella chejuensis, a marine-derived microorganism, has several biological functions, including antimicrobial activity and inflammatory relief. In this study, the antibacterial activity of prodigiosin against skin microorganisms was explored. Paper disc assay on skin bacterial cells revealed that Cutibacterium acnes related to acne vulgaris highly susceptible to prodigiosin. MIC (Minimal Inhibitory Concentration) and MBC (Minimal Bactericidal Concentration) were determined on Cutibacterium species. The RNA-seq analysis of prodigiosin-treated C. acnes cells was performed to understand the antibacterial mechanism of prodigiosin. Among changes in the expression of hundreds of genes, the expression of a stress-responsive sigma factor encoded by sigB increased. Conversely, the gene expression of cell wall biosynthesis and energy metabolism was inhibited by prodigiosin. Specifically, the expression of genes related to the metabolism of porphyrin, a pro-inflammatory metabolite, was significantly reduced. Therefore, prodigiosin could be used to control C. acnes. Our study provided new insights into the antimicrobial mechanism of prodigiosin against C. acnes strains.
灵菌红素是一种由海洋来源的微生物海鞘素产生的红色色素，具有多种生物学功能，包括抗菌活性和炎症缓解作用。在本研究中，探索了灵菌红素对皮肤微生物的抗菌活性。纸片法检测皮肤细菌细胞表明，与寻常痤疮相关的痤疮丙酸杆菌对灵菌红素高度敏感。测定了灵菌红素对痤疮丙酸杆菌属的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。对灵菌红素处理的痤疮丙酸杆菌细胞进行 RNA-seq 分析，以了解灵菌红素的抗菌机制。在数百个基因表达变化中，编码应激响应σ因子 sigB 的基因表达增加。相反，灵菌红素抑制细胞壁生物合成和能量代谢的基因表达。具体而言，与卟啉代谢相关的基因表达，一种促炎代谢物，显著降低。因此，灵菌红素可用于控制痤疮丙酸杆菌。本研究为灵菌红素对痤疮丙酸杆菌菌株的抗菌机制提供了新的见解。
