Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, China.
Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.
Int J Mol Sci. 2023 Sep 25;24(19):14518. doi: 10.3390/ijms241914518.
Salt stress severely restricts plant growth and crop production, which is accompanied by accumulation of reactive oxygen species (ROS) that disturb cell redox homeostasis and oxidize redox-sensitive proteins. , a halophytic species closely related to , shows a high level of tolerance to salinity and is increasingly used as a model plant in abiotic stress biology. To understand redox modifications and signaling pathways under salt stress, we used tandem mass tag (TMT)-based proteomics to quantify the salt-induced changes in protein redox modifications in . Salt stress led to increased oxidative modification levels of 159 cysteine sites in 107 proteins, which play roles in carbohydrate and energy metabolism, transport, ROS homeostasis, cellular structure modulation, and folding and assembly. These lists of unknown redox reactive proteins in salt mustard lay the foundation for future research to understand the molecular mechanism of plant salt response. However, glutathione peroxidase (GPX) is one of the most important antioxidant enzymes in plants. Our research indicates that may be involved in regulating ROS levels and that plants with overexpressed have much improved salt tolerance.
盐胁迫严重限制了植物的生长和作物产量，同时伴随着活性氧（ROS）的积累，破坏了细胞氧化还原平衡，氧化了氧化还原敏感蛋白。作为一种与 密切相关的盐生植物，表现出对盐度的高度耐受性，并且越来越多地被用作非生物胁迫生物学的模式植物。为了了解盐胁迫下的氧化还原修饰和信号通路，我们使用串联质量标签（TMT）基于蛋白质组学来定量盐胁迫下 中蛋白质氧化还原修饰的变化。盐胁迫导致 107 种蛋白质中 159 个半胱氨酸位点的氧化修饰水平增加，这些蛋白质参与碳水化合物和能量代谢、运输、ROS 稳态、细胞结构调节以及折叠和组装。这些盐芥中未知的氧化还原反应蛋白列表为未来研究植物盐响应的分子机制奠定了基础。然而，谷胱甘肽过氧化物酶（GPX）是植物中最重要的抗氧化酶之一。我们的研究表明 可能参与调节 ROS 水平，并且过表达 的植物具有更高的耐盐性。
