Sun Hongmei, Wang Yong, Cao Lianbin, Wang Yihan, Wei Zhaoyang, Song Li, Jiang Jun, Liu Junhe, Tian Shilin
School of Biological and Food Engineering, Huanghuai University, Zhumadian, Henan, China.
Vegetable Research Institute, Zhumadian Academy of Agricultural Sciences, Zhumadian, Henan, China.
BMC Genomics. 2025 Jul 4;26(1):635. doi: 10.1186/s12864-025-11802-8.
Roots are the initial organs that perceive and adaptively adjust when exposed to salt stress. However, the mechanisms underlying salt stress tolerance in eggplant (Solanum melongena) roots remain elusive. In this study, salt stress markedly elevated the Na ion concentration, reactive oxygen species (ROS), proline, and malondialdehyde (MDA) content, in addition to enhancing the activities of superoxide dismutase (SOD) and peroxidase (POD) in the roots of the eggplant inbred ZH171. Transcriptome analysis revealed a total of 3491 DEGs in eggplant roots response to salt stress. Most of the DEGs under salt stress were mainly involved in oxidoreductase activity, hydrolase activity, pentose and glucuronate interconversion, microtubule motor activity, ion transport, hydrolytic activity. Some genes were enriched in peroxidase activity, indicating the activation of the antioxidant enzyme system in alleviating oxidative damage under salt stress. A total of 236 DEGs mainly within ERF, MYB, NAC, bHLH, and WRKY TF families, and the most upregulated genes from NAC. Taken together, this study provides important candidate genes linked to salt tolerance in eggplant roots, offering a foundation for further in-depth study of resistance mechanisms in eggplant ZH171.
根是植物在遭受盐胁迫时最先感知并进行适应性调节的器官。然而，茄子（Solanum melongena）根系耐盐胁迫的机制仍不清楚。在本研究中，盐胁迫显著提高了茄子自交系ZH171根系中的钠离子浓度、活性氧（ROS）、脯氨酸和丙二醛（MDA）含量，同时增强了超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性。转录组分析显示，茄子根系对盐胁迫有响应的差异表达基因（DEG）共有3491个。盐胁迫下的大多数DEG主要参与氧化还原酶活性、水解酶活性、戊糖与葡糖醛酸相互转化、微管运动活性、离子运输、水解活性。一些基因在过氧化物酶活性中富集，表明抗氧化酶系统被激活以减轻盐胁迫下的氧化损伤。共有236个DEG主要分布在ERF、MYB、NAC、bHLH和WRKY转录因子家族中，其中NAC家族中上调基因最多。综上所述，本研究提供了与茄子根系耐盐性相关的重要候选基因，为进一步深入研究茄子ZH171的抗性机制奠定了基础。
