Palmer Nathan A, Alvarez Sophie, Naldrett Michael J, Muhle Anthony, Sarath Gautam, Edmé Serge J, Tatineni Satyanarayana, Mitchell Robert B, Yuen Gary
Wheat, Sorghum, and Forage Research Unit, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Lincoln, NE 68583-0937, USA.
Proteomics and Metabolomics Core Facility, Center for Biotechnology, University of Nebraska at Lincoln, Lincoln, NE 68588-0664, USA.
Int J Mol Sci. 2023 Sep 27;24(19):14630. doi: 10.3390/ijms241914630.
Switchgrass ( L.) can be infected by the rust pathogen () and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 proteins were identified at 1% false discovery rate, with 3632 mapped to the switchgrass proteome and 198 proteins mapped to different proteomes. Across all comparisons, 1825 differentially accumulated switchgrass proteins were identified and subjected to a STRING analysis using Arabidopsis ( L.) orthologs to deduce switchgrass cellular pathways impacted by rust infection. Proteins associated with plastid functions and primary metabolism were diminished in infected Summer plants at all harvest dates, whereas proteins associated with immunity, chaperone functions, and phenylpropanoid biosynthesis were significantly enriched. At 18 DAI, 1105 and 151 proteins were significantly enriched or diminished, respectively. Many of the enriched proteins were associated with mitigation of cellular stress and defense.
柳枝稷（Panicum virgatum L.）可被锈病病原体（Puccinia emaculata）感染，导致生物量产量和质量下降。对接种后7天、11天和18天从易感品种（Summer）的未感染和感染植株上采集的叶片提取物进行了无标记定量蛋白质组学分析，以跟踪病害进展并评估植物对感染的任何补偿机制。在接种后7天可见一些脓疱，其数量随时间增加。然而，在该实验过程中，感染植株中的真菌DNA含量没有明显变化。总共以1%的错误发现率鉴定出3830种蛋白质，其中3632种映射到柳枝稷蛋白质组，198种蛋白质映射到不同的蛋白质组。在所有比较中，鉴定出1825种差异积累的柳枝稷蛋白质，并使用拟南芥（Arabidopsis thaliana L.）直系同源物进行STRING分析，以推断受锈病感染影响的柳枝稷细胞途径。在所有收获日期，感染的Summer植株中与质体功能和初级代谢相关的蛋白质减少，而与免疫、伴侣功能和苯丙烷生物合成相关的蛋白质显著富集。在接种后18天，分别有1105种和151种蛋白质显著富集或减少。许多富集的蛋白质与减轻细胞应激和防御有关。
